Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain

M Brand, C P Heisenberg, Y J Jiang, D Beuchle, K Lun, Makoto Furutani-Seiki, M Granato, P Haffter, M Hammerschmidt, D A Kane, R N Kelsh, M C Mullins, J Odenthal, F J M vanEeden, C NussleinVolhard

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Abstract

Mutations in two genes affect the formation of the boundary between midbrain and hindbrain (MHB): no isthmus (noi) and acerebellar (ace), noi mutant embryos lack the MHB constriction, the cerebellum and optic tectum, as well as the pronephric duct. Analysis of noi mutant embryos with neuron-specific antibodies shows that the MHB region and the dorsal and ventral midbrain are absent or abnormal, but that the rostral hindbrain is unaffected with the exception of the cerebellum, Using markers that are expressed during its formation (eng, wnt1 and pax-b), we find that the MHB region is already misspecified in noi mutant embryos during late gastrulation. The tectum is initially present and later degenerates, The defect in ace mutant embryos is more restricted: MHB and cerebellum are absent, but a tectum is formed, Molecular organisation of the tectum and tegmentum is disturbed, however, since eng, wntl and pax-b marker gene expression is not maintained, We propose that noi and ace are required for development of the MHB region and of the adjacent mid- and hindbrain, which are thought to be patterned by the MHB region, Presence of pax-b RNA, and absence of pax-b protein, together with the observation of genetic linkage and the occurrence of a point mutation, show that noi mutations are located in the pax-b gene, pax-b is a vertebrate orthologue of the Drosophila gene paired, which is involved in a pathway of cellular interactions at the posterior compartment boundary in Drosophila, Our results confirm and extend a previous report, and show that at least one member of this conserved signalling pathway is required for formation of the boundary between midbrain and hindbrain in the zebrafish.
LanguageEnglish
Pages179-190
Number of pages12
JournalDevelopment
Volume123
StatusPublished - 1996

Fingerprint

Rhombencephalon
Zebrafish
Mesencephalon
Mutation
Genes
Embryonic Structures
Cerebellum
Drosophila
Gastrulation
Genetic Linkage
Superior Colliculi
Point Mutation
Constriction
Vertebrates
Observation
RNA
Gene Expression
Neurons

Keywords

  • pax genes
  • acerebellar
  • Danio rerio
  • hindbrain
  • neurogenesis
  • no
  • midbrain
  • isthmus
  • zebrafrsh
  • regionalization
  • rhombencephali
  • neural development

Cite this

Brand, M., Heisenberg, C. P., Jiang, Y. J., Beuchle, D., Lun, K., Furutani-Seiki, M., ... NussleinVolhard, C. (1996). Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain.

Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain. / Brand, M; Heisenberg, C P; Jiang, Y J; Beuchle, D; Lun, K; Furutani-Seiki, Makoto; Granato, M; Haffter, P; Hammerschmidt, M; Kane, D A; Kelsh, R N; Mullins, M C; Odenthal, J; vanEeden, F J M; NussleinVolhard, C.

In: Development, Vol. 123, 1996, p. 179-190.

Research output: Contribution to journalArticle

Brand, M, Heisenberg, CP, Jiang, YJ, Beuchle, D, Lun, K, Furutani-Seiki, M, Granato, M, Haffter, P, Hammerschmidt, M, Kane, DA, Kelsh, RN, Mullins, MC, Odenthal, J, vanEeden, FJM & NussleinVolhard, C 1996, 'Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain' Development, vol. 123, pp. 179-190.
Brand M, Heisenberg CP, Jiang YJ, Beuchle D, Lun K, Furutani-Seiki M et al. Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain. Development. 1996;123:179-190.
Brand, M ; Heisenberg, C P ; Jiang, Y J ; Beuchle, D ; Lun, K ; Furutani-Seiki, Makoto ; Granato, M ; Haffter, P ; Hammerschmidt, M ; Kane, D A ; Kelsh, R N ; Mullins, M C ; Odenthal, J ; vanEeden, F J M ; NussleinVolhard, C. / Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain. In: Development. 1996 ; Vol. 123. pp. 179-190
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abstract = "Mutations in two genes affect the formation of the boundary between midbrain and hindbrain (MHB): no isthmus (noi) and acerebellar (ace), noi mutant embryos lack the MHB constriction, the cerebellum and optic tectum, as well as the pronephric duct. Analysis of noi mutant embryos with neuron-specific antibodies shows that the MHB region and the dorsal and ventral midbrain are absent or abnormal, but that the rostral hindbrain is unaffected with the exception of the cerebellum, Using markers that are expressed during its formation (eng, wnt1 and pax-b), we find that the MHB region is already misspecified in noi mutant embryos during late gastrulation. The tectum is initially present and later degenerates, The defect in ace mutant embryos is more restricted: MHB and cerebellum are absent, but a tectum is formed, Molecular organisation of the tectum and tegmentum is disturbed, however, since eng, wntl and pax-b marker gene expression is not maintained, We propose that noi and ace are required for development of the MHB region and of the adjacent mid- and hindbrain, which are thought to be patterned by the MHB region, Presence of pax-b RNA, and absence of pax-b protein, together with the observation of genetic linkage and the occurrence of a point mutation, show that noi mutations are located in the pax-b gene, pax-b is a vertebrate orthologue of the Drosophila gene paired, which is involved in a pathway of cellular interactions at the posterior compartment boundary in Drosophila, Our results confirm and extend a previous report, and show that at least one member of this conserved signalling pathway is required for formation of the boundary between midbrain and hindbrain in the zebrafish.",
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AU - Brand,M

AU - Heisenberg,C P

AU - Jiang,Y J

AU - Beuchle,D

AU - Lun,K

AU - Furutani-Seiki,Makoto

AU - Granato,M

AU - Haffter,P

AU - Hammerschmidt,M

AU - Kane,D A

AU - Kelsh,R N

AU - Mullins,M C

AU - Odenthal,J

AU - vanEeden,F J M

AU - NussleinVolhard,C

PY - 1996

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N2 - Mutations in two genes affect the formation of the boundary between midbrain and hindbrain (MHB): no isthmus (noi) and acerebellar (ace), noi mutant embryos lack the MHB constriction, the cerebellum and optic tectum, as well as the pronephric duct. Analysis of noi mutant embryos with neuron-specific antibodies shows that the MHB region and the dorsal and ventral midbrain are absent or abnormal, but that the rostral hindbrain is unaffected with the exception of the cerebellum, Using markers that are expressed during its formation (eng, wnt1 and pax-b), we find that the MHB region is already misspecified in noi mutant embryos during late gastrulation. The tectum is initially present and later degenerates, The defect in ace mutant embryos is more restricted: MHB and cerebellum are absent, but a tectum is formed, Molecular organisation of the tectum and tegmentum is disturbed, however, since eng, wntl and pax-b marker gene expression is not maintained, We propose that noi and ace are required for development of the MHB region and of the adjacent mid- and hindbrain, which are thought to be patterned by the MHB region, Presence of pax-b RNA, and absence of pax-b protein, together with the observation of genetic linkage and the occurrence of a point mutation, show that noi mutations are located in the pax-b gene, pax-b is a vertebrate orthologue of the Drosophila gene paired, which is involved in a pathway of cellular interactions at the posterior compartment boundary in Drosophila, Our results confirm and extend a previous report, and show that at least one member of this conserved signalling pathway is required for formation of the boundary between midbrain and hindbrain in the zebrafish.

AB - Mutations in two genes affect the formation of the boundary between midbrain and hindbrain (MHB): no isthmus (noi) and acerebellar (ace), noi mutant embryos lack the MHB constriction, the cerebellum and optic tectum, as well as the pronephric duct. Analysis of noi mutant embryos with neuron-specific antibodies shows that the MHB region and the dorsal and ventral midbrain are absent or abnormal, but that the rostral hindbrain is unaffected with the exception of the cerebellum, Using markers that are expressed during its formation (eng, wnt1 and pax-b), we find that the MHB region is already misspecified in noi mutant embryos during late gastrulation. The tectum is initially present and later degenerates, The defect in ace mutant embryos is more restricted: MHB and cerebellum are absent, but a tectum is formed, Molecular organisation of the tectum and tegmentum is disturbed, however, since eng, wntl and pax-b marker gene expression is not maintained, We propose that noi and ace are required for development of the MHB region and of the adjacent mid- and hindbrain, which are thought to be patterned by the MHB region, Presence of pax-b RNA, and absence of pax-b protein, together with the observation of genetic linkage and the occurrence of a point mutation, show that noi mutations are located in the pax-b gene, pax-b is a vertebrate orthologue of the Drosophila gene paired, which is involved in a pathway of cellular interactions at the posterior compartment boundary in Drosophila, Our results confirm and extend a previous report, and show that at least one member of this conserved signalling pathway is required for formation of the boundary between midbrain and hindbrain in the zebrafish.

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KW - acerebellar

KW - Danio rerio

KW - hindbrain

KW - neurogenesis

KW - no

KW - midbrain

KW - isthmus

KW - zebrafrsh

KW - regionalization

KW - rhombencephali

KW - neural development

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VL - 123

SP - 179

EP - 190

JO - Development

T2 - Development

JF - Development

SN - 0950-1991

ER -