Mutations in the translation initiation region of the pac gene resulting in increased levels of activity of penicillin G acylase

Özlem Akkaya, Saliha İşsever Öztürk, Albert Bolhuis, Füsun Gümüşel

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Abstract

Penicillin G acylase (PA) is an important enzyme used in the industrial production of b-lactam antibiotics. In this study, the effects of mutations in the translation initiation region of the Escherichia coli pac gene, encoding periplasmic PA, were examined. Several mutations led to increased amounts of PA activity, including those that lengthened the spacer region between the ribosome binding site and the ATG start codon, and those with altered codons on positions +2 and +4 relative to the start codon. These results indicated that the wild-type sequence of the pac gene does not provide maximum expression levels and that the strategies applied in this study can be used to improve production of PA in E. coli. Unexpectedly, our study also suggested that translocation of PA was, in contrast to earlier reports, shown not to require the Twin-arginine translocation pathway for transport into the periplasm.
Original languageEnglish
Pages (from-to)2159-2164
Number of pages6
JournalWorld Journal of Microbiology and Biotechnology
Volume28
Issue number5
DOIs
Publication statusPublished - 2012

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