Mutations affecting the cardiovascular system and other internal organs in zebrafish

J N Chen, P Haffter, J Odenthal, E Vogelsang, M Brand, F J M vanEeden, Makoto Furutani-Seiki, M Granato, M Hammerschmidt, C P Heisenberg, Y J Jiang, D A Kane, R N Kelsh, M C Mullins, C NussleinVolhard

Research output: Contribution to journalArticle

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Abstract

In a screen for early developmental mutants of the zebrafish, we have identified mutations specifically affecting the internal organs, We identified 53 mutations affecting the cardiovascular system, Nine of them affect specific landmarks of heart morphogenesis. Mutations in four genes cause a failure in the fusion of the bilateral heart primordia, resulting in cardia bifida. In lonely atrium, no heart venticle is visible and the atrium is directly fused to the outflow tract. In the overlooped mutant, the relative position of the two heart chambers is distorted, The heart is enormously enlarged in the santa mutant, In two mutants, scotch tape and superglue, the cardiac jelly between the two layers of the heart is significantly reduced, We also identified a number of mutations affecting the function of the heart, The mutations affecting heart function can be subdivided into two groups, one affecting heart contraction and another affecting the rhythm of the heart beat. Among the contractility group of mutants are 5 with no heart beat at all and 15 with a reduced heart beat of one or both chambers, 6 mutations are in the rhythmicity group and specifically affect the beating pattern of the heart, Mutations in two genes, bypass and kurzschluss, cause specific defects in the circulatory system, In addition to the heart mutants, we identified 23 mutations affecting the integrity of the liver, the intestine or the kidney, In this report, we demonstrate that it is feasible to screen for genes specific for the patterning or function of certain internal organs in the zebrafish, The mutations presented here could serve as an entrypoint to the establishment of a genetic hierarchy underlying organogenesis.
LanguageEnglish
Pages293-302
Number of pages10
JournalDevelopment
Volume123
StatusPublished - 1996

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Zebrafish
Cardiovascular System
Mutation
Genes
Cardia
Organogenesis
Periodicity
Morphogenesis
Intestines

Keywords

  • cardiovascular system
  • heart
  • circulation
  • liver
  • intestine
  • zebrafish
  • kidney

Cite this

Chen, J. N., Haffter, P., Odenthal, J., Vogelsang, E., Brand, M., vanEeden, F. J. M., ... NussleinVolhard, C. (1996). Mutations affecting the cardiovascular system and other internal organs in zebrafish.

Mutations affecting the cardiovascular system and other internal organs in zebrafish. / Chen, J N; Haffter, P; Odenthal, J; Vogelsang, E; Brand, M; vanEeden, F J M; Furutani-Seiki, Makoto; Granato, M; Hammerschmidt, M; Heisenberg, C P; Jiang, Y J; Kane, D A; Kelsh, R N; Mullins, M C; NussleinVolhard, C.

In: Development, Vol. 123, 1996, p. 293-302.

Research output: Contribution to journalArticle

Chen, JN, Haffter, P, Odenthal, J, Vogelsang, E, Brand, M, vanEeden, FJM, Furutani-Seiki, M, Granato, M, Hammerschmidt, M, Heisenberg, CP, Jiang, YJ, Kane, DA, Kelsh, RN, Mullins, MC & NussleinVolhard, C 1996, 'Mutations affecting the cardiovascular system and other internal organs in zebrafish' Development, vol. 123, pp. 293-302.
Chen JN, Haffter P, Odenthal J, Vogelsang E, Brand M, vanEeden FJM et al. Mutations affecting the cardiovascular system and other internal organs in zebrafish. Development. 1996;123:293-302.
Chen, J N ; Haffter, P ; Odenthal, J ; Vogelsang, E ; Brand, M ; vanEeden, F J M ; Furutani-Seiki, Makoto ; Granato, M ; Hammerschmidt, M ; Heisenberg, C P ; Jiang, Y J ; Kane, D A ; Kelsh, R N ; Mullins, M C ; NussleinVolhard, C. / Mutations affecting the cardiovascular system and other internal organs in zebrafish. In: Development. 1996 ; Vol. 123. pp. 293-302
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AU - Odenthal,J

AU - Vogelsang,E

AU - Brand,M

AU - vanEeden,F J M

AU - Furutani-Seiki,Makoto

AU - Granato,M

AU - Hammerschmidt,M

AU - Heisenberg,C P

AU - Jiang,Y J

AU - Kane,D A

AU - Kelsh,R N

AU - Mullins,M C

AU - NussleinVolhard,C

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N2 - In a screen for early developmental mutants of the zebrafish, we have identified mutations specifically affecting the internal organs, We identified 53 mutations affecting the cardiovascular system, Nine of them affect specific landmarks of heart morphogenesis. Mutations in four genes cause a failure in the fusion of the bilateral heart primordia, resulting in cardia bifida. In lonely atrium, no heart venticle is visible and the atrium is directly fused to the outflow tract. In the overlooped mutant, the relative position of the two heart chambers is distorted, The heart is enormously enlarged in the santa mutant, In two mutants, scotch tape and superglue, the cardiac jelly between the two layers of the heart is significantly reduced, We also identified a number of mutations affecting the function of the heart, The mutations affecting heart function can be subdivided into two groups, one affecting heart contraction and another affecting the rhythm of the heart beat. Among the contractility group of mutants are 5 with no heart beat at all and 15 with a reduced heart beat of one or both chambers, 6 mutations are in the rhythmicity group and specifically affect the beating pattern of the heart, Mutations in two genes, bypass and kurzschluss, cause specific defects in the circulatory system, In addition to the heart mutants, we identified 23 mutations affecting the integrity of the liver, the intestine or the kidney, In this report, we demonstrate that it is feasible to screen for genes specific for the patterning or function of certain internal organs in the zebrafish, The mutations presented here could serve as an entrypoint to the establishment of a genetic hierarchy underlying organogenesis.

AB - In a screen for early developmental mutants of the zebrafish, we have identified mutations specifically affecting the internal organs, We identified 53 mutations affecting the cardiovascular system, Nine of them affect specific landmarks of heart morphogenesis. Mutations in four genes cause a failure in the fusion of the bilateral heart primordia, resulting in cardia bifida. In lonely atrium, no heart venticle is visible and the atrium is directly fused to the outflow tract. In the overlooped mutant, the relative position of the two heart chambers is distorted, The heart is enormously enlarged in the santa mutant, In two mutants, scotch tape and superglue, the cardiac jelly between the two layers of the heart is significantly reduced, We also identified a number of mutations affecting the function of the heart, The mutations affecting heart function can be subdivided into two groups, one affecting heart contraction and another affecting the rhythm of the heart beat. Among the contractility group of mutants are 5 with no heart beat at all and 15 with a reduced heart beat of one or both chambers, 6 mutations are in the rhythmicity group and specifically affect the beating pattern of the heart, Mutations in two genes, bypass and kurzschluss, cause specific defects in the circulatory system, In addition to the heart mutants, we identified 23 mutations affecting the integrity of the liver, the intestine or the kidney, In this report, we demonstrate that it is feasible to screen for genes specific for the patterning or function of certain internal organs in the zebrafish, The mutations presented here could serve as an entrypoint to the establishment of a genetic hierarchy underlying organogenesis.

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KW - circulation

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