Mutations affecting pigmentation and shape of the adult zebrafish

P Haffter, J Odenthal, M C Mullins, S Lin, M J Farrell, E Vogelsang, F Haas, M Brand, F J M vanEeden, Makoto Furutani-Seiki, M Granato, M Hammerschmidt, C P Heisenberg, Y J Jiang, D A Kane, R N Kelsh, N Hopkins, C NussleinVolhard

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Mutations causing a visible phenotype in the adult serve as valuable visible genetic markers in multicellular genetic model organisms such as Drosophila melanogaster, Caenorhabditis elegans and Arabidopsis thaliana. In a large scale screen for mutations affecting early development of the zebrafish, we identified a number of mutations that are homozygous viable or semiviable. Here we describe viable mutations which produce visible phenotypes in the adult fish. These predominantly affect the fins and pigmentation, but also the eyes and body length of the adult. A number of dominant mutations caused visible phenotypes in the adult fish, Mutations in three genes, long fin, another long fin and wanda affected fin formation in the adult. Four mutations were found to cause a dominant reduction of the overall body length in the adult. The adult pigment pattern was found to be changed by dominant mutations in wanda, asterix, obelix, leopard, salz and pfeffer. Among the recessive mutations producing visible phenotypes in the homozygous adult, a group of mutations that failed to produce melanin was assayed for tyrosinase activity. Mutations in sandy produced embryos that failed to express tyrosinase activity. These are potentially useful for using tyrosinase as a marker for the generation of transgenic lines of zebrafish.
Original languageEnglish
Pages (from-to)260-276
Number of pages17
JournalDevelopment Genes and Evolution
Volume206
Issue number4
Publication statusPublished - 1996

Fingerprint

Pigmentation
Zebrafish
Danio rerio
pigmentation
mutation
Mutation
fins
Monophenol Monooxygenase
phenotype
Phenotype
body length
Fishes
Panthera
melanin
Genetic Models
Melanins
Caenorhabditis elegans
genetic marker
fish
Drosophila melanogaster

Keywords

  • fish skeleton
  • tyrosinase
  • fin
  • pigment pattern
  • zebrafish

Cite this

Haffter, P., Odenthal, J., Mullins, M. C., Lin, S., Farrell, M. J., Vogelsang, E., ... NussleinVolhard, C. (1996). Mutations affecting pigmentation and shape of the adult zebrafish. Development Genes and Evolution, 206(4), 260-276.

Mutations affecting pigmentation and shape of the adult zebrafish. / Haffter, P; Odenthal, J; Mullins, M C; Lin, S; Farrell, M J; Vogelsang, E; Haas, F; Brand, M; vanEeden, F J M; Furutani-Seiki, Makoto; Granato, M; Hammerschmidt, M; Heisenberg, C P; Jiang, Y J; Kane, D A; Kelsh, R N; Hopkins, N; NussleinVolhard, C.

In: Development Genes and Evolution, Vol. 206, No. 4, 1996, p. 260-276.

Research output: Contribution to journalArticle

Haffter, P, Odenthal, J, Mullins, MC, Lin, S, Farrell, MJ, Vogelsang, E, Haas, F, Brand, M, vanEeden, FJM, Furutani-Seiki, M, Granato, M, Hammerschmidt, M, Heisenberg, CP, Jiang, YJ, Kane, DA, Kelsh, RN, Hopkins, N & NussleinVolhard, C 1996, 'Mutations affecting pigmentation and shape of the adult zebrafish', Development Genes and Evolution, vol. 206, no. 4, pp. 260-276.
Haffter P, Odenthal J, Mullins MC, Lin S, Farrell MJ, Vogelsang E et al. Mutations affecting pigmentation and shape of the adult zebrafish. Development Genes and Evolution. 1996;206(4):260-276.
Haffter, P ; Odenthal, J ; Mullins, M C ; Lin, S ; Farrell, M J ; Vogelsang, E ; Haas, F ; Brand, M ; vanEeden, F J M ; Furutani-Seiki, Makoto ; Granato, M ; Hammerschmidt, M ; Heisenberg, C P ; Jiang, Y J ; Kane, D A ; Kelsh, R N ; Hopkins, N ; NussleinVolhard, C. / Mutations affecting pigmentation and shape of the adult zebrafish. In: Development Genes and Evolution. 1996 ; Vol. 206, No. 4. pp. 260-276.
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AU - Odenthal, J

AU - Mullins, M C

AU - Lin, S

AU - Farrell, M J

AU - Vogelsang, E

AU - Haas, F

AU - Brand, M

AU - vanEeden, F J M

AU - Furutani-Seiki, Makoto

AU - Granato, M

AU - Hammerschmidt, M

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AB - Mutations causing a visible phenotype in the adult serve as valuable visible genetic markers in multicellular genetic model organisms such as Drosophila melanogaster, Caenorhabditis elegans and Arabidopsis thaliana. In a large scale screen for mutations affecting early development of the zebrafish, we identified a number of mutations that are homozygous viable or semiviable. Here we describe viable mutations which produce visible phenotypes in the adult fish. These predominantly affect the fins and pigmentation, but also the eyes and body length of the adult. A number of dominant mutations caused visible phenotypes in the adult fish, Mutations in three genes, long fin, another long fin and wanda affected fin formation in the adult. Four mutations were found to cause a dominant reduction of the overall body length in the adult. The adult pigment pattern was found to be changed by dominant mutations in wanda, asterix, obelix, leopard, salz and pfeffer. Among the recessive mutations producing visible phenotypes in the homozygous adult, a group of mutations that failed to produce melanin was assayed for tyrosinase activity. Mutations in sandy produced embryos that failed to express tyrosinase activity. These are potentially useful for using tyrosinase as a marker for the generation of transgenic lines of zebrafish.

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