Mutations affecting development of the zebrafish inner ear and lateral line

T T Whitfield, M Granato, F J M vanEeden, U Schach, M Brand, Makoto Furutani-Seiki, P Haffter, M Hammerschmidt, C P Heisenberg, Y J Jiang, D A Kane, R N Kelsh, M C Mullins, J Odenthal, C NussleinVolhard

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Abstract

Mutations giving rise to anatomical defects in the inner ear have been isolated in a large scale screen for mutations causing visible abnormalities in the zebrafish embryo (Haffter, P., Granato, M., Brand, M. et al. (1996) Development 123, 1-36). 58 mutants have been classified as having a primary ear phenotype; these fall into several phenotypic classes, affecting presence or size of the otoliths, size and shape of the otic vesicle and formation of the semicircular canals, and define at least 20 complementation groups. Mutations in seven genes cause loss of one or both otoliths, but do not appear to affect development of other structures within the ear. Mutations in seven genes affect morphology and patterning of the inner ear epithelium, including formation of the semicircular canals and, in some, development of sensory patches (maculae and cristae). Within this class, dog-eared mutants show abnormal development of semicircular canals and lack cristae within the ear, while in van gogh, semicircular canals fail to form altogether, resulting in a tiny otic vesicle containing a single sensory patch. Both these mutants show defects in the expression of homeobox genes within the otic vesicle. In a further class of mutants, ear size is affected while patterning appears to be relatively normal; mutations in three genes cause expansion of the otic vesicle, while in little ears and microtic, the ear is abnormally small, but still contains all five sensory patches, as in the wild type. Many of the ear and otolith mutants show an expected behavioural phenotype: embryos fail to balance correctly, and may swim on their sides, upside down, or in circles. Several mutants with similar balance defects have also been isolated that have no obvious structural ear defect, but that may include mutants with vestibular dysfunction of the inner ear (Granato, M., van Eeden, F. J. M., Schach, U. et al. (1996) Development, 123, 399-413,). Mutations in 19 genes causing primary defects in other structures also show an ear defect. In particular, ear phenotypes are often found in conjunction with defects of neural crest derivatives (pigment cells and/or cartilaginous elements of the jaw). At least one mutant, dog-eared, shows defects in both the ear and another placodally derived sensory system, the lateral line, while hypersensitive mutants have additional trunk lateral line organs.
Original languageEnglish
Pages (from-to)241-254
Number of pages14
JournalDevelopment
Volume123
Publication statusPublished - 1996

Fingerprint

Zebrafish
Inner Ear
Ear
Mutation
Semicircular Canals
Otolithic Membrane
Lateral Line System
Phenotype
Genes
Embryonic Structures
Neural Crest
Homeobox Genes
Jaw

Keywords

  • hearing
  • semicircular canal
  • Danio rerio
  • balance
  • lateral line
  • otolith
  • inner ear
  • otic vesicle
  • mutant
  • zebrafish
  • mechanosensory
  • hair cell

Cite this

Whitfield, T. T., Granato, M., vanEeden, F. J. M., Schach, U., Brand, M., Furutani-Seiki, M., ... NussleinVolhard, C. (1996). Mutations affecting development of the zebrafish inner ear and lateral line. Development, 123, 241-254.

Mutations affecting development of the zebrafish inner ear and lateral line. / Whitfield, T T; Granato, M; vanEeden, F J M; Schach, U; Brand, M; Furutani-Seiki, Makoto; Haffter, P; Hammerschmidt, M; Heisenberg, C P; Jiang, Y J; Kane, D A; Kelsh, R N; Mullins, M C; Odenthal, J; NussleinVolhard, C.

In: Development, Vol. 123, 1996, p. 241-254.

Research output: Contribution to journalArticle

Whitfield, TT, Granato, M, vanEeden, FJM, Schach, U, Brand, M, Furutani-Seiki, M, Haffter, P, Hammerschmidt, M, Heisenberg, CP, Jiang, YJ, Kane, DA, Kelsh, RN, Mullins, MC, Odenthal, J & NussleinVolhard, C 1996, 'Mutations affecting development of the zebrafish inner ear and lateral line', Development, vol. 123, pp. 241-254.
Whitfield TT, Granato M, vanEeden FJM, Schach U, Brand M, Furutani-Seiki M et al. Mutations affecting development of the zebrafish inner ear and lateral line. Development. 1996;123:241-254.
Whitfield, T T ; Granato, M ; vanEeden, F J M ; Schach, U ; Brand, M ; Furutani-Seiki, Makoto ; Haffter, P ; Hammerschmidt, M ; Heisenberg, C P ; Jiang, Y J ; Kane, D A ; Kelsh, R N ; Mullins, M C ; Odenthal, J ; NussleinVolhard, C. / Mutations affecting development of the zebrafish inner ear and lateral line. In: Development. 1996 ; Vol. 123. pp. 241-254.
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abstract = "Mutations giving rise to anatomical defects in the inner ear have been isolated in a large scale screen for mutations causing visible abnormalities in the zebrafish embryo (Haffter, P., Granato, M., Brand, M. et al. (1996) Development 123, 1-36). 58 mutants have been classified as having a primary ear phenotype; these fall into several phenotypic classes, affecting presence or size of the otoliths, size and shape of the otic vesicle and formation of the semicircular canals, and define at least 20 complementation groups. Mutations in seven genes cause loss of one or both otoliths, but do not appear to affect development of other structures within the ear. Mutations in seven genes affect morphology and patterning of the inner ear epithelium, including formation of the semicircular canals and, in some, development of sensory patches (maculae and cristae). Within this class, dog-eared mutants show abnormal development of semicircular canals and lack cristae within the ear, while in van gogh, semicircular canals fail to form altogether, resulting in a tiny otic vesicle containing a single sensory patch. Both these mutants show defects in the expression of homeobox genes within the otic vesicle. In a further class of mutants, ear size is affected while patterning appears to be relatively normal; mutations in three genes cause expansion of the otic vesicle, while in little ears and microtic, the ear is abnormally small, but still contains all five sensory patches, as in the wild type. Many of the ear and otolith mutants show an expected behavioural phenotype: embryos fail to balance correctly, and may swim on their sides, upside down, or in circles. Several mutants with similar balance defects have also been isolated that have no obvious structural ear defect, but that may include mutants with vestibular dysfunction of the inner ear (Granato, M., van Eeden, F. J. M., Schach, U. et al. (1996) Development, 123, 399-413,). Mutations in 19 genes causing primary defects in other structures also show an ear defect. In particular, ear phenotypes are often found in conjunction with defects of neural crest derivatives (pigment cells and/or cartilaginous elements of the jaw). At least one mutant, dog-eared, shows defects in both the ear and another placodally derived sensory system, the lateral line, while hypersensitive mutants have additional trunk lateral line organs.",
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T1 - Mutations affecting development of the zebrafish inner ear and lateral line

AU - Whitfield, T T

AU - Granato, M

AU - vanEeden, F J M

AU - Schach, U

AU - Brand, M

AU - Furutani-Seiki, Makoto

AU - Haffter, P

AU - Hammerschmidt, M

AU - Heisenberg, C P

AU - Jiang, Y J

AU - Kane, D A

AU - Kelsh, R N

AU - Mullins, M C

AU - Odenthal, J

AU - NussleinVolhard, C

PY - 1996

Y1 - 1996

N2 - Mutations giving rise to anatomical defects in the inner ear have been isolated in a large scale screen for mutations causing visible abnormalities in the zebrafish embryo (Haffter, P., Granato, M., Brand, M. et al. (1996) Development 123, 1-36). 58 mutants have been classified as having a primary ear phenotype; these fall into several phenotypic classes, affecting presence or size of the otoliths, size and shape of the otic vesicle and formation of the semicircular canals, and define at least 20 complementation groups. Mutations in seven genes cause loss of one or both otoliths, but do not appear to affect development of other structures within the ear. Mutations in seven genes affect morphology and patterning of the inner ear epithelium, including formation of the semicircular canals and, in some, development of sensory patches (maculae and cristae). Within this class, dog-eared mutants show abnormal development of semicircular canals and lack cristae within the ear, while in van gogh, semicircular canals fail to form altogether, resulting in a tiny otic vesicle containing a single sensory patch. Both these mutants show defects in the expression of homeobox genes within the otic vesicle. In a further class of mutants, ear size is affected while patterning appears to be relatively normal; mutations in three genes cause expansion of the otic vesicle, while in little ears and microtic, the ear is abnormally small, but still contains all five sensory patches, as in the wild type. Many of the ear and otolith mutants show an expected behavioural phenotype: embryos fail to balance correctly, and may swim on their sides, upside down, or in circles. Several mutants with similar balance defects have also been isolated that have no obvious structural ear defect, but that may include mutants with vestibular dysfunction of the inner ear (Granato, M., van Eeden, F. J. M., Schach, U. et al. (1996) Development, 123, 399-413,). Mutations in 19 genes causing primary defects in other structures also show an ear defect. In particular, ear phenotypes are often found in conjunction with defects of neural crest derivatives (pigment cells and/or cartilaginous elements of the jaw). At least one mutant, dog-eared, shows defects in both the ear and another placodally derived sensory system, the lateral line, while hypersensitive mutants have additional trunk lateral line organs.

AB - Mutations giving rise to anatomical defects in the inner ear have been isolated in a large scale screen for mutations causing visible abnormalities in the zebrafish embryo (Haffter, P., Granato, M., Brand, M. et al. (1996) Development 123, 1-36). 58 mutants have been classified as having a primary ear phenotype; these fall into several phenotypic classes, affecting presence or size of the otoliths, size and shape of the otic vesicle and formation of the semicircular canals, and define at least 20 complementation groups. Mutations in seven genes cause loss of one or both otoliths, but do not appear to affect development of other structures within the ear. Mutations in seven genes affect morphology and patterning of the inner ear epithelium, including formation of the semicircular canals and, in some, development of sensory patches (maculae and cristae). Within this class, dog-eared mutants show abnormal development of semicircular canals and lack cristae within the ear, while in van gogh, semicircular canals fail to form altogether, resulting in a tiny otic vesicle containing a single sensory patch. Both these mutants show defects in the expression of homeobox genes within the otic vesicle. In a further class of mutants, ear size is affected while patterning appears to be relatively normal; mutations in three genes cause expansion of the otic vesicle, while in little ears and microtic, the ear is abnormally small, but still contains all five sensory patches, as in the wild type. Many of the ear and otolith mutants show an expected behavioural phenotype: embryos fail to balance correctly, and may swim on their sides, upside down, or in circles. Several mutants with similar balance defects have also been isolated that have no obvious structural ear defect, but that may include mutants with vestibular dysfunction of the inner ear (Granato, M., van Eeden, F. J. M., Schach, U. et al. (1996) Development, 123, 399-413,). Mutations in 19 genes causing primary defects in other structures also show an ear defect. In particular, ear phenotypes are often found in conjunction with defects of neural crest derivatives (pigment cells and/or cartilaginous elements of the jaw). At least one mutant, dog-eared, shows defects in both the ear and another placodally derived sensory system, the lateral line, while hypersensitive mutants have additional trunk lateral line organs.

KW - hearing

KW - semicircular canal

KW - Danio rerio

KW - balance

KW - lateral line

KW - otolith

KW - inner ear

KW - otic vesicle

KW - mutant

KW - zebrafish

KW - mechanosensory

KW - hair cell

M3 - Article

VL - 123

SP - 241

EP - 254

JO - Development

JF - Development

SN - 0950-1991

ER -