Mutations affecting development of the midline and general body shape during zebrafish embryogenesis

M Brand, C P Heisenberg, R M Warga, F Pelegri, R O Karlstrom, D Beuchle, A Picker, Y J Jiang, Makoto Furutani-Seiki, F J M vanEeden, M Granato, P Haffter, M Hammerschmidt, D A Kane, R N Kelsh, M C Mullins, J Odenthal, C NussleinVolhard

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

Tissues of the dorsal midline of vertebrate embryos, such as notochord and floor plate, have been implicated in inductive interactions that pattern the neural tube and somites. In our screen for embryonic visible mutations in the zebrafish we found 113 mutations in more than 27 genes with altered body shape, often with additional defects in CNS development. We concentrated on a subgroup of mutations in ten genes (the midline-group) that cause defective development of the floor plate. By using floor plate markers, such as the signaling molecule sonic hedgehog, we show that the schmalspur (sur) gene is needed for early floor plate development, similar to one-eyed-pinhead (oep) and the previously described cyclops (eye) gene. In contrast to oep and cyc, sur embryos show deletions of ventral CNS tissue restricted to the mid- and hindbrain, whereas the forebrain appears largely unaffected. In the underlying mesendodermal tissue of the head, sur is needed only for development of the posterior prechordal plate, whereas oep and eye are required for both anterior and posterior prechordal plate development. Our analysis of sur mutants suggests that defects within the posterior prechordal plate may cause aberrant development of ventral CNS structures in the mid- and hindbrain. Later development of the floor plate is affected in mutant chameleon, you-too, sonic-you, iguana, detour, schmalkars and monorail embryos; these mutants often show additional defects in tissues that are known to depend on signals from notochord and floor plate, For example, sur, con, and yot mutants show reduction of motor neurons; median deletions of brain tissue are seen in sur, con and yot embryos; and eye, con, yet, igu and dtr mutants often show no or abnormal formation of the optic chiasm. We also find fusions of the ventral neurocranium for all midline mutants tested, which may reveal a hitherto unrecognized function of the midline in influencing differentiation of neural crest cells at their destination. As a working hypothesis, we propose that midline-group genes may act to maintain proper structure and inductive function of zebrafish midline tissues.
Original languageEnglish
Pages (from-to)129-142
Number of pages14
JournalDevelopment
Volume123
Publication statusPublished - 1996

Fingerprint

Zebrafish
Embryonic Development
Mutation
Embryonic Structures
Notochord
Rhombencephalon
Genes
Mesencephalon
Iguanas
Optic Chiasm
Somites
Minocycline
Neural Tube
Lizards
Hedgehogs
Neural Crest
Motor Neurons
Prosencephalon
Vertebrates
Head

Keywords

  • Danio rerio
  • floor
  • neurogenesis
  • neural induction
  • regionalisation
  • plate
  • body shape
  • zebrafish
  • neurocranium
  • neuronal development

Cite this

Brand, M., Heisenberg, C. P., Warga, R. M., Pelegri, F., Karlstrom, R. O., Beuchle, D., ... NussleinVolhard, C. (1996). Mutations affecting development of the midline and general body shape during zebrafish embryogenesis. Development, 123, 129-142.

Mutations affecting development of the midline and general body shape during zebrafish embryogenesis. / Brand, M; Heisenberg, C P; Warga, R M; Pelegri, F; Karlstrom, R O; Beuchle, D; Picker, A; Jiang, Y J; Furutani-Seiki, Makoto; vanEeden, F J M; Granato, M; Haffter, P; Hammerschmidt, M; Kane, D A; Kelsh, R N; Mullins, M C; Odenthal, J; NussleinVolhard, C.

In: Development, Vol. 123, 1996, p. 129-142.

Research output: Contribution to journalArticle

Brand, M, Heisenberg, CP, Warga, RM, Pelegri, F, Karlstrom, RO, Beuchle, D, Picker, A, Jiang, YJ, Furutani-Seiki, M, vanEeden, FJM, Granato, M, Haffter, P, Hammerschmidt, M, Kane, DA, Kelsh, RN, Mullins, MC, Odenthal, J & NussleinVolhard, C 1996, 'Mutations affecting development of the midline and general body shape during zebrafish embryogenesis', Development, vol. 123, pp. 129-142.
Brand M, Heisenberg CP, Warga RM, Pelegri F, Karlstrom RO, Beuchle D et al. Mutations affecting development of the midline and general body shape during zebrafish embryogenesis. Development. 1996;123:129-142.
Brand, M ; Heisenberg, C P ; Warga, R M ; Pelegri, F ; Karlstrom, R O ; Beuchle, D ; Picker, A ; Jiang, Y J ; Furutani-Seiki, Makoto ; vanEeden, F J M ; Granato, M ; Haffter, P ; Hammerschmidt, M ; Kane, D A ; Kelsh, R N ; Mullins, M C ; Odenthal, J ; NussleinVolhard, C. / Mutations affecting development of the midline and general body shape during zebrafish embryogenesis. In: Development. 1996 ; Vol. 123. pp. 129-142.
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abstract = "Tissues of the dorsal midline of vertebrate embryos, such as notochord and floor plate, have been implicated in inductive interactions that pattern the neural tube and somites. In our screen for embryonic visible mutations in the zebrafish we found 113 mutations in more than 27 genes with altered body shape, often with additional defects in CNS development. We concentrated on a subgroup of mutations in ten genes (the midline-group) that cause defective development of the floor plate. By using floor plate markers, such as the signaling molecule sonic hedgehog, we show that the schmalspur (sur) gene is needed for early floor plate development, similar to one-eyed-pinhead (oep) and the previously described cyclops (eye) gene. In contrast to oep and cyc, sur embryos show deletions of ventral CNS tissue restricted to the mid- and hindbrain, whereas the forebrain appears largely unaffected. In the underlying mesendodermal tissue of the head, sur is needed only for development of the posterior prechordal plate, whereas oep and eye are required for both anterior and posterior prechordal plate development. Our analysis of sur mutants suggests that defects within the posterior prechordal plate may cause aberrant development of ventral CNS structures in the mid- and hindbrain. Later development of the floor plate is affected in mutant chameleon, you-too, sonic-you, iguana, detour, schmalkars and monorail embryos; these mutants often show additional defects in tissues that are known to depend on signals from notochord and floor plate, For example, sur, con, and yot mutants show reduction of motor neurons; median deletions of brain tissue are seen in sur, con and yot embryos; and eye, con, yet, igu and dtr mutants often show no or abnormal formation of the optic chiasm. We also find fusions of the ventral neurocranium for all midline mutants tested, which may reveal a hitherto unrecognized function of the midline in influencing differentiation of neural crest cells at their destination. As a working hypothesis, we propose that midline-group genes may act to maintain proper structure and inductive function of zebrafish midline tissues.",
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T1 - Mutations affecting development of the midline and general body shape during zebrafish embryogenesis

AU - Brand, M

AU - Heisenberg, C P

AU - Warga, R M

AU - Pelegri, F

AU - Karlstrom, R O

AU - Beuchle, D

AU - Picker, A

AU - Jiang, Y J

AU - Furutani-Seiki, Makoto

AU - vanEeden, F J M

AU - Granato, M

AU - Haffter, P

AU - Hammerschmidt, M

AU - Kane, D A

AU - Kelsh, R N

AU - Mullins, M C

AU - Odenthal, J

AU - NussleinVolhard, C

PY - 1996

Y1 - 1996

N2 - Tissues of the dorsal midline of vertebrate embryos, such as notochord and floor plate, have been implicated in inductive interactions that pattern the neural tube and somites. In our screen for embryonic visible mutations in the zebrafish we found 113 mutations in more than 27 genes with altered body shape, often with additional defects in CNS development. We concentrated on a subgroup of mutations in ten genes (the midline-group) that cause defective development of the floor plate. By using floor plate markers, such as the signaling molecule sonic hedgehog, we show that the schmalspur (sur) gene is needed for early floor plate development, similar to one-eyed-pinhead (oep) and the previously described cyclops (eye) gene. In contrast to oep and cyc, sur embryos show deletions of ventral CNS tissue restricted to the mid- and hindbrain, whereas the forebrain appears largely unaffected. In the underlying mesendodermal tissue of the head, sur is needed only for development of the posterior prechordal plate, whereas oep and eye are required for both anterior and posterior prechordal plate development. Our analysis of sur mutants suggests that defects within the posterior prechordal plate may cause aberrant development of ventral CNS structures in the mid- and hindbrain. Later development of the floor plate is affected in mutant chameleon, you-too, sonic-you, iguana, detour, schmalkars and monorail embryos; these mutants often show additional defects in tissues that are known to depend on signals from notochord and floor plate, For example, sur, con, and yot mutants show reduction of motor neurons; median deletions of brain tissue are seen in sur, con and yot embryos; and eye, con, yet, igu and dtr mutants often show no or abnormal formation of the optic chiasm. We also find fusions of the ventral neurocranium for all midline mutants tested, which may reveal a hitherto unrecognized function of the midline in influencing differentiation of neural crest cells at their destination. As a working hypothesis, we propose that midline-group genes may act to maintain proper structure and inductive function of zebrafish midline tissues.

AB - Tissues of the dorsal midline of vertebrate embryos, such as notochord and floor plate, have been implicated in inductive interactions that pattern the neural tube and somites. In our screen for embryonic visible mutations in the zebrafish we found 113 mutations in more than 27 genes with altered body shape, often with additional defects in CNS development. We concentrated on a subgroup of mutations in ten genes (the midline-group) that cause defective development of the floor plate. By using floor plate markers, such as the signaling molecule sonic hedgehog, we show that the schmalspur (sur) gene is needed for early floor plate development, similar to one-eyed-pinhead (oep) and the previously described cyclops (eye) gene. In contrast to oep and cyc, sur embryos show deletions of ventral CNS tissue restricted to the mid- and hindbrain, whereas the forebrain appears largely unaffected. In the underlying mesendodermal tissue of the head, sur is needed only for development of the posterior prechordal plate, whereas oep and eye are required for both anterior and posterior prechordal plate development. Our analysis of sur mutants suggests that defects within the posterior prechordal plate may cause aberrant development of ventral CNS structures in the mid- and hindbrain. Later development of the floor plate is affected in mutant chameleon, you-too, sonic-you, iguana, detour, schmalkars and monorail embryos; these mutants often show additional defects in tissues that are known to depend on signals from notochord and floor plate, For example, sur, con, and yot mutants show reduction of motor neurons; median deletions of brain tissue are seen in sur, con and yot embryos; and eye, con, yet, igu and dtr mutants often show no or abnormal formation of the optic chiasm. We also find fusions of the ventral neurocranium for all midline mutants tested, which may reveal a hitherto unrecognized function of the midline in influencing differentiation of neural crest cells at their destination. As a working hypothesis, we propose that midline-group genes may act to maintain proper structure and inductive function of zebrafish midline tissues.

KW - Danio rerio

KW - floor

KW - neurogenesis

KW - neural induction

KW - regionalisation

KW - plate

KW - body shape

KW - zebrafish

KW - neurocranium

KW - neuronal development

M3 - Article

VL - 123

SP - 129

EP - 142

JO - Development

JF - Development

SN - 0950-1991

ER -