Mutants of Pseudomonas fluorescens NCIMB 11671 defective in the catabolism of α-pinene

A. Colocousi, K. M. Saqib, D. J. Leak

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Pseudomonas fluorescens NCIMB 11671 metabolises α-pinene via α-pinene oxide and 2-methyl-5-isopropylhexa-2,5-dienal. Mutants unable to grow on α- pinene and/or α-pinene oxide have been isolated by N-methyl-N'-nitro-N- nitrosoguanidine mutagenesis, including an unexpected phenotype able to grow on α-pinene but not on α-pinene oxide. The mutants have been classified on the basis of their α-pinene monooxygenase, α-pinene oxide lyase and aldehyde dehydrogenase activities. Biotransformation of α-pinene by the wild-type and mutant strains has revealed evidence for alternative routes for pinene metabolism to that already proposed.

Original languageEnglish
Pages (from-to)822-830
Number of pages9
JournalApplied Microbiology and Biotechnology
Issue number6
Publication statusPublished - 1 Jan 1996

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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