Mutant resources for functional genomics in Dictyostelium discoideum using REMI-seq technology

Nicole Gruenheit, Amy Baldwin, Balint Stewart, Sarah Jaques, Thomas Keller, Katie Parkinson, William Salvidge, Robert Baines, Chris Brimson, Jason B. Wolf, Rex Chisholm, Adrian J. Harwood, Christopher R.L. Thompson

Research output: Contribution to journalArticlepeer-review

11 Citations (SciVal)


Background: Genomes can be sequenced with relative ease, but ascribing gene function remains a major challenge. Genetically tractable model systems are crucial to meet this challenge. One powerful model is the social amoeba Dictyostelium discoideum, a eukaryotic microbe widely used to study diverse questions in the cell, developmental and evolutionary biology. Results: We describe REMI-seq, an adaptation of Tn-seq, which allows high throughput, en masse, and quantitative identification of the genomic site of insertion of a drug resistance marker after restriction enzyme-mediated integration. We use REMI-seq to develop tools which greatly enhance the efficiency with which the sequence, transcriptome or proteome variation can be linked to phenotype in D. discoideum. These comprise (1) a near genome-wide resource of individual mutants and (2) a defined pool of ‘barcoded’ mutants to allow large-scale parallel phenotypic analyses. These resources are freely available and easily accessible through the REMI-seq website that also provides comprehensive guidance and pipelines for data analysis. We demonstrate that integrating these resources allows novel regulators of cell migration, phagocytosis and macropinocytosis to be rapidly identified. Conclusions: We present methods and resources, generated using REMI-seq, for high throughput gene function analysis in a key model system.

Original languageEnglish
Article number172
JournalBMC Biology
Issue number1
Early online date24 Aug 2021
Publication statusPublished - 31 Dec 2021


  • Dictyostelium discoideum
  • Functional genomics
  • Genome-wide mutant resource
  • Parallel phenotypic analysis
  • REMI-seq

ASJC Scopus subject areas

  • Biotechnology
  • Structural Biology
  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Plant Science
  • Developmental Biology
  • Cell Biology


Dive into the research topics of 'Mutant resources for functional genomics in Dictyostelium discoideum using REMI-seq technology'. Together they form a unique fingerprint.

Cite this