Multimodal electrochemical and nanoplasmonic biosensors using ferrocene crowned nanoparticles for kinase drug discovery applications

Nello Formisano, Nikhil Bhalla, Lai Chun Caleb Wong, Mirella Di Lorenzo, Giordano Pula, Pedro Estrela

Research output: Contribution to journalArticle

12 Citations (Scopus)
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Abstract

The use of on-chip multimodal sensing approaches is very promising towards integrated biosensing systems, which measure different parameters involved in biomolecular interactions and provide automated validation of true positives. In this report we investigate a proof of concept that enables multiple detection technologies for screening inhibitors of kinase activity, which is a crucial process in drug discovery applications. We demonstrate the integration of electrochemical techniques on the same chip, namely differential pulse voltammetry, impedance spectroscopy and direct open circuit potential measurements. Gold nanoparticles that attach to the thio-phosphorylated proteins facilitate localized surface plasmon resonance detection. The addition of thiolated ferrocene, which attaches to the nanoparticles like a crown, enables sensitive electrochemical amperometric detection of kinase activity. This novel multimodal biosensor provides a more rigorous measurement of biomolecules, with wide significance in biomedical, environmental and pharmaceutical applications.
Original languageEnglish
Pages (from-to)70-73
Number of pages4
JournalElectrochemistry Communications
Volume57
Early online date29 May 2015
DOIs
Publication statusPublished - Aug 2015

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Biosensors
Phosphotransferases
Nanoparticles
Surface plasmon resonance
Biomolecules
Voltammetry
Gold
Drug products
Screening
Spectroscopy
Proteins
Networks (circuits)
Pharmaceutical Preparations
ferrocene
Drug Discovery

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Multimodal electrochemical and nanoplasmonic biosensors using ferrocene crowned nanoparticles for kinase drug discovery applications. / Formisano, Nello; Bhalla, Nikhil; Wong, Lai Chun Caleb; Di Lorenzo, Mirella; Pula, Giordano; Estrela, Pedro.

In: Electrochemistry Communications, Vol. 57, 08.2015, p. 70-73.

Research output: Contribution to journalArticle

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