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Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men

  • A. Birtles
  • , N. McCarthy
  • , C. L. Sheppard
  • , H. Rutter
  • , M. Guiver
  • , E. Haworth
  • , R. C. George
  • Health Protection Agency
  • Public Health England

Research output: Contribution to journalArticlepeer-review

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Abstract

Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.
Original languageEnglish
Pages (from-to)2004-2008
Number of pages5
JournalJournal of Clinical Microbiology
Volume43
Issue number4
DOIs
Publication statusPublished - 2005

Bibliographical note

Birtles, Andrew McCarthy, Noel Sheppard, Carmen L Rutter, Harry Guiver, Malcolm Haworth, Elizabeth George, Robert C eng Case Reports Research Support, Non-U.S. Gov't 2005/04/09 09:00 J Clin Microbiol. 2005 Apr;43(4):2004-8.

Keywords

  • Adult Bacterial Proteins/*genetics Bacterial Typing Techniques Blood/microbiology Cerebrospinal Fluid/microbiology Fatal Outcome *Homeless Persons Humans Male Meningitis, Pneumococcal/*microbiology Polymerase Chain Reaction *Sequence Analysis, DNA Streptococcus pneumoniae/*classification/genetics/isolation & purification Streptolysins/*genetics

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