Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men

A. Birtles, N. McCarthy, C. L. Sheppard, H. Rutter, M. Guiver, E. Haworth, R. C. George

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Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.
Original languageEnglish
Pages (from-to)2004-2008
Number of pages5
JournalJournal of Clinical Microbiology
Issue number4
Publication statusPublished - 2005


  • Adult Bacterial Proteins/*genetics Bacterial Typing Techniques Blood/microbiology Cerebrospinal Fluid/microbiology Fatal Outcome *Homeless Persons Humans Male Meningitis, Pneumococcal/*microbiology Polymerase Chain Reaction *Sequence Analysis, DNA Streptococcus pneumoniae/*classification/genetics/isolation & purification Streptolysins/*genetics

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