Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men

A. Birtles, N. McCarthy, C. L. Sheppard, H. Rutter, M. Guiver, E. Haworth, R. C. George

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.
Original languageEnglish
Pages (from-to)2004-2008
Number of pages5
JournalJournal of Clinical Microbiology
Volume43
Issue number4
DOIs
Publication statusPublished - 2005

Keywords

  • Adult Bacterial Proteins/*genetics Bacterial Typing Techniques Blood/microbiology Cerebrospinal Fluid/microbiology Fatal Outcome *Homeless Persons Humans Male Meningitis, Pneumococcal/*microbiology Polymerase Chain Reaction *Sequence Analysis, DNA Streptococcus pneumoniae/*classification/genetics/isolation & purification Streptolysins/*genetics

Cite this

Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men. / Birtles, A.; McCarthy, N.; Sheppard, C. L.; Rutter, H.; Guiver, M.; Haworth, E.; George, R. C.

In: Journal of Clinical Microbiology, Vol. 43, No. 4, 2005, p. 2004-2008.

Research output: Contribution to journalArticle

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abstract = "Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.",
keywords = "Adult Bacterial Proteins/*genetics Bacterial Typing Techniques Blood/microbiology Cerebrospinal Fluid/microbiology Fatal Outcome *Homeless Persons Humans Male Meningitis, Pneumococcal/*microbiology Polymerase Chain Reaction *Sequence Analysis, DNA Streptococcus pneumoniae/*classification/genetics/isolation & purification Streptolysins/*genetics",
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AU - Birtles, A.

AU - McCarthy, N.

AU - Sheppard, C. L.

AU - Rutter, H.

AU - Guiver, M.

AU - Haworth, E.

AU - George, R. C.

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