Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.
- Adult Bacterial Proteins/*genetics Bacterial Typing Techniques Blood/microbiology Cerebrospinal Fluid/microbiology Fatal Outcome *Homeless Persons Humans Male Meningitis, Pneumococcal/*microbiology Polymerase Chain Reaction *Sequence Analysis, DNA Streptococcus pneumoniae/*classification/genetics/isolation & purification Streptolysins/*genetics
Birtles, A., McCarthy, N., Sheppard, C. L., Rutter, H., Guiver, M., Haworth, E., & George, R. C. (2005). Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men. Journal of Clinical Microbiology, 43(4), 2004-2008. https://doi.org/10.1128/JCM.43.4.2004-2008.2005