Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii

Gabriele Margos, A Hojgaard, R S Lane, M Cornet, V Fingerle, N Rudenko, N Ogden, D M Aanensen, D Fish, J Piesman

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Using multilocus sequence analysis (MLSA), we investigated the phylogenetic relationship of spirochaete strains from North America previously assigned to the genospecies Borrelia bissettii. We amplified internal fragments of 8 housekeeping genes (clpA, clpX, nifS, pepX, pyrG, recG, rplB, and uvrA) located on the main linear chromosome by polymerase chain reaction. Phylogenetic analysis of concatenated sequences of the 8 lad showed that the B. bissettii clade consisted of 4 closely related clusters which included strains from California (including the type strain DN127-C19-2/p7) and Colorado that were isolated from Ixodes pacificus, I. spinipalpis, or infected reservoir hosts. Several strains isolated from I. scapularis clustered distantly from B. bissettii. Genetic distance analyses confirmed that these strains are more distant to B. bissettii than B. carolinensis, a recently described Borrelia species, which suggests that they constitute a new Borrelia genospecies. We propose that it be named Borrelia kurtenbachii sp. nov. in honour of the late Klaus Kurtenbach. The data suggest that ecological differences between B. bissettii and the new Borrelia genospecies reflect different transmission cycles. In view of these findings, the distinct vertebrate host-tick vector associations and the distributions of B. bissettii and B. kurtenbachii require further investigation.
LanguageEnglish
Pages151-158
Number of pages8
JournalTicks and Tick-Borne Diseases
Volume1
Issue number4
DOIs
StatusPublished - Dec 2010

Fingerprint

Borrelia bissettii
Borrelia
Multilocus Sequence Typing
North America
Ixodes
Spirochaetales
Ixodes pacificus
Essential Genes
Ticks
Ixodes scapularis
disease reservoirs
phylogeny
Sequence Analysis
Vertebrates
Chromosomes
genetic distance
ticks
multilocus sequence typing
Polymerase Chain Reaction
polymerase chain reaction

Keywords

  • Ixodes
  • molecular ecology
  • multilocus sequence analysis
  • Borrelia kurtenbachii sp nov.
  • Borrelia bissettii

Cite this

Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii. / Margos, Gabriele; Hojgaard, A; Lane, R S; Cornet, M; Fingerle, V; Rudenko, N; Ogden, N; Aanensen, D M; Fish, D; Piesman, J.

In: Ticks and Tick-Borne Diseases, Vol. 1, No. 4, 12.2010, p. 151-158.

Research output: Contribution to journalArticle

Margos, G, Hojgaard, A, Lane, RS, Cornet, M, Fingerle, V, Rudenko, N, Ogden, N, Aanensen, DM, Fish, D & Piesman, J 2010, 'Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii', Ticks and Tick-Borne Diseases, vol. 1, no. 4, pp. 151-158. https://doi.org/10.1016/j.ttbdis.2010.09.002
Margos, Gabriele ; Hojgaard, A ; Lane, R S ; Cornet, M ; Fingerle, V ; Rudenko, N ; Ogden, N ; Aanensen, D M ; Fish, D ; Piesman, J. / Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii. In: Ticks and Tick-Borne Diseases. 2010 ; Vol. 1, No. 4. pp. 151-158.
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