TY - JOUR
T1 - Multi-locus sequence typing of Bartonella henselae isolates from three continents reveals hypervirulent and feline-associated clones
AU - Arvand, Mardjan
AU - Feil, Edward J.
AU - Giladi, Michael
AU - Boulouis, Henri Jean
AU - Viezens, Juliane
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/12/19
Y1 - 2007/12/19
N2 - Bartonella henselae is a zoonotic pathogen and the causative agent of cat scratch disease and a variety of other disease manifestations in humans. Previous investigations have suggested that a limited subset of B. henselae isolates may be associated with human disease. In the present study, 182 human and feline B. henselaeisolates from Europe, North America and Australia were analysed by multi-locus sequence typing (MLST) to detect any associations between sequence type (ST), host species and geographical distribution of the isolates. A total of 14 sequence types were detected, but over 66% (16/24) of the isolates recovered from human disease corresponded to a single genotype, ST1, and this type was detected in all three continents. In contrast, 27.2% (43/ 158) of the feline isolates corresponded to ST7, but this ST was not recovered from humans and was restricted to Europe. The difference in host association of STs 1 (human) and 7 (feline) was statistically significant (P≤0.001). eBURST analysis assigned the 14 STs to three clonal lineages, which contained two or more STs, and a singleton comprising ST7. These groups were broadly consistent with a neighbour-joining tree, although splits decomposition analysis was indicative of a history of recombination. These data indicate that B. henselaelineages differ in their virulence properties for humans and contribute to a better understanding of the population structure of B. henselae.
AB - Bartonella henselae is a zoonotic pathogen and the causative agent of cat scratch disease and a variety of other disease manifestations in humans. Previous investigations have suggested that a limited subset of B. henselae isolates may be associated with human disease. In the present study, 182 human and feline B. henselaeisolates from Europe, North America and Australia were analysed by multi-locus sequence typing (MLST) to detect any associations between sequence type (ST), host species and geographical distribution of the isolates. A total of 14 sequence types were detected, but over 66% (16/24) of the isolates recovered from human disease corresponded to a single genotype, ST1, and this type was detected in all three continents. In contrast, 27.2% (43/ 158) of the feline isolates corresponded to ST7, but this ST was not recovered from humans and was restricted to Europe. The difference in host association of STs 1 (human) and 7 (feline) was statistically significant (P≤0.001). eBURST analysis assigned the 14 STs to three clonal lineages, which contained two or more STs, and a singleton comprising ST7. These groups were broadly consistent with a neighbour-joining tree, although splits decomposition analysis was indicative of a history of recombination. These data indicate that B. henselaelineages differ in their virulence properties for humans and contribute to a better understanding of the population structure of B. henselae.
UR - http://www.scopus.com/inward/record.url?scp=44349123303&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0001346
DO - 10.1371/journal.pone.0001346
M3 - Article
C2 - 18094753
AN - SCOPUS:44349123303
VL - 2
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 12
M1 - e1346
ER -