Mono-functional aminosilanes as primers for peptide functionalization

Elise C Pegg, Gavin S Walker, Colin A Scotchford, David Farrar, David Grant

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
187 Downloads (Pure)

Abstract

When covalently attaching biomolecules to surfaces such as titanium, trifunctional silanes are commonly used as primers to produce surface amine groups. However, these primed surfaces are rarely uniform in structure due to networking of the silane. Mono-functional aminosilanes may result in more uniform structures, although their long-term stability and effect on osteoblast cell responses are possible issues for orthopedic applications. This study examines for the first time the optimization of peptide coupling to titanium using mono-functional aminosilane reaction chemistry. The resultant surface topography, chemistry, and thicknesses were characterized showing improved surface uniformity compared with trifunctional silanized surfaces. The stability of the coatings was examined over a period of 8 days in environments of varying pH, temperature, and humidity. In addition, human osteosarcoma (HOS) cell adhesion and spreading on the samples was examined; adhesion was minimal on silanized surfaces, but after functionalization with cysteine the cell density was greater than the titanium control and showed no overall detrimental effect on initial cell responses.

Original languageEnglish
Pages (from-to)947-58
Number of pages12
JournalJournal of Biomedical Materials Research - Part A
Volume90
Issue number4
DOIs
Publication statusPublished - 15 Sep 2009

Keywords

  • Cell Adhesion
  • Biomaterials
  • Coated Materials
  • Cysteine
  • Titanium
  • Joint Prosthesis
  • Osteoblast
  • Peptides
  • Silanes

Fingerprint Dive into the research topics of 'Mono-functional aminosilanes as primers for peptide functionalization'. Together they form a unique fingerprint.

Cite this