TY - JOUR
T1 - Monitoring salivary testosterone and cortisol concentrations across an international sports competition
T2 - Data comparison using two enzyme immunoassays and two sample preparations
AU - Crewther, B.T.
AU - Al-Dujaili, E.
AU - Smail, N.F.
AU - Anastasova, S.
AU - Kilduff, L.P.
AU - Cook, C.J.
PY - 2013/3
Y1 - 2013/3
N2 - Objectives: Salivary testosterone (T) and cortisol (C) concentrations were monitored across a sports competition. Data were compared using two enzyme-immunoassay (EIA) methods and two sample preparations to determine their influence on hormone concentrations. Design and methods: A group of male athletes (n=19) provided a saliva sample the morning before and one day after (24h post) an international rugby union match. Following an extraction procedure, the samples were analysed for T and C concentrations using a commercial kit (CM) and an in-house method (IH). Raw samples (no extraction procedure) were also tested using the commercial kit (CM). Results: There were no significant changes in T and C levels from pre to post competition with each EIA method and sample preparation, but significant differences in T (IH>CM>CM) and C (CM>IH and CM) concentrations were seen when both samples were pooled. Bland-Altman analyses confirmed the presence of fixed and proportional bias. Strong and significant correlations were demonstrated between the IH and CM measures of salivary T (r=0.93-0.97) and C (r=0.95-0.97). The T and C values from the raw and extracted samples were also strongly correlated (r=0.93-0.96). Conclusions: The measurement of salivary T and C concentrations across an international sports event was influenced by different EIA methods and sample preparations, but all measures were strongly correlated with some bias. Both T and C were unresponsive to the sports event, but within the group results large individual variation was seen.
AB - Objectives: Salivary testosterone (T) and cortisol (C) concentrations were monitored across a sports competition. Data were compared using two enzyme-immunoassay (EIA) methods and two sample preparations to determine their influence on hormone concentrations. Design and methods: A group of male athletes (n=19) provided a saliva sample the morning before and one day after (24h post) an international rugby union match. Following an extraction procedure, the samples were analysed for T and C concentrations using a commercial kit (CM) and an in-house method (IH). Raw samples (no extraction procedure) were also tested using the commercial kit (CM). Results: There were no significant changes in T and C levels from pre to post competition with each EIA method and sample preparation, but significant differences in T (IH>CM>CM) and C (CM>IH and CM) concentrations were seen when both samples were pooled. Bland-Altman analyses confirmed the presence of fixed and proportional bias. Strong and significant correlations were demonstrated between the IH and CM measures of salivary T (r=0.93-0.97) and C (r=0.95-0.97). The T and C values from the raw and extracted samples were also strongly correlated (r=0.93-0.96). Conclusions: The measurement of salivary T and C concentrations across an international sports event was influenced by different EIA methods and sample preparations, but all measures were strongly correlated with some bias. Both T and C were unresponsive to the sports event, but within the group results large individual variation was seen.
UR - http://www.scopus.com/inward/record.url?scp=84873724056&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1016/j.clinbiochem.2012.11.019
U2 - 10.1016/j.clinbiochem.2012.11.019
DO - 10.1016/j.clinbiochem.2012.11.019
M3 - Article
AN - SCOPUS:84873724056
VL - 46
SP - 354
EP - 358
JO - Clinical Biochemistry
JF - Clinical Biochemistry
SN - 0009-9120
IS - 4-5
ER -