Monitoring changes in thioredoxin and over-oxidised peroxiredoxin in response to exercise in humans

A. J. Wadley, Y. W. Chen, S. J. Bennett, G. Y. H. Lip, J. E. Turner, J. P. Fisher, S. Aldred

Research output: Contribution to journalArticle

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Abstract

Abstract Introduction: Peroxiredoxin (PRDX) and Thioredoxin (TRX) are antioxidant proteins that control cellular signalling and redox balance, although their response to exercise is unknown. This study aimed to assess key aspects of the PRDX-TRX redox cycle in response to three different modes of exercise. Methods: Healthy males (n=10, mean ± SD: age 22 ± 3 yrs) undertook three exercise trials on separate days: two steady-state cycling trials at a moderate (60% MAX; 27 min, MOD) and high (80% MAX; 20 min, HIGH) intensity, and a low volume high intensity interval training trial (10×1 min 90% MAX, LV-HIIT). Peripheral blood mononuclear cells (PBMCs) were assessed for TRX-1 and over-oxidised PRDX (isoforms I-IV) protein expression before, during and 30 minutes following exercise (post+30). The activities of TRX reductase (TRX-R) and the NF-κB p65 subunit were also assessed. Results: TRX-1 increased during exercise in all trials (MOD +84.5%; HIGH +64.1%; LV-HIIT +205.7%; p<.05), whereas over-oxidised PRDX increased during HIGH only (MOD -28.7%; HIGH +202.9%; LV-HIIT -22.7%; p<.05). TRX-R and NF-κB p65 activity increased during exercise in all trials, with the greatest response in TRX-R activity seen in HIGH (p<.05). Discussion: All trials stimulated a transient increase in TRX-1 protein expression during exercise. Only HIGH induced a transient over-oxidation of PRDX, alongside the greatest change in TRX-R activity. Future studies are needed to clarify the significance of heightened peroxide exposure during continuous high intensity exercise and the mechanisms of PRDX-regulatory control.
LanguageEnglish
Pages290-298
JournalFree Radical Research
Volume49
Issue number3
Early online date4 Feb 2015
DOIs
StatusPublished - Mar 2015

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Peroxiredoxins
Thioredoxins
Monitoring
Oxidation-Reduction
Thioredoxin-Disulfide Reductase
Cell signaling
Proteins
Peroxides
Blood Cells
Protein Isoforms
Blood
Antioxidants
Oxidation

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Monitoring changes in thioredoxin and over-oxidised peroxiredoxin in response to exercise in humans. / Wadley, A. J.; Chen, Y. W.; Bennett, S. J.; Lip, G. Y. H.; Turner, J. E.; Fisher, J. P.; Aldred, S.

In: Free Radical Research, Vol. 49, No. 3, 03.2015, p. 290-298.

Research output: Contribution to journalArticle

Wadley, A. J. ; Chen, Y. W. ; Bennett, S. J. ; Lip, G. Y. H. ; Turner, J. E. ; Fisher, J. P. ; Aldred, S. / Monitoring changes in thioredoxin and over-oxidised peroxiredoxin in response to exercise in humans. In: Free Radical Research. 2015 ; Vol. 49, No. 3. pp. 290-298.
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abstract = "Abstract Introduction: Peroxiredoxin (PRDX) and Thioredoxin (TRX) are antioxidant proteins that control cellular signalling and redox balance, although their response to exercise is unknown. This study aimed to assess key aspects of the PRDX-TRX redox cycle in response to three different modes of exercise. Methods: Healthy males (n=10, mean ± SD: age 22 ± 3 yrs) undertook three exercise trials on separate days: two steady-state cycling trials at a moderate (60{\%} MAX; 27 min, MOD) and high (80{\%} MAX; 20 min, HIGH) intensity, and a low volume high intensity interval training trial (10×1 min 90{\%} MAX, LV-HIIT). Peripheral blood mononuclear cells (PBMCs) were assessed for TRX-1 and over-oxidised PRDX (isoforms I-IV) protein expression before, during and 30 minutes following exercise (post+30). The activities of TRX reductase (TRX-R) and the NF-κB p65 subunit were also assessed. Results: TRX-1 increased during exercise in all trials (MOD +84.5{\%}; HIGH +64.1{\%}; LV-HIIT +205.7{\%}; p<.05), whereas over-oxidised PRDX increased during HIGH only (MOD -28.7{\%}; HIGH +202.9{\%}; LV-HIIT -22.7{\%}; p<.05). TRX-R and NF-κB p65 activity increased during exercise in all trials, with the greatest response in TRX-R activity seen in HIGH (p<.05). Discussion: All trials stimulated a transient increase in TRX-1 protein expression during exercise. Only HIGH induced a transient over-oxidation of PRDX, alongside the greatest change in TRX-R activity. Future studies are needed to clarify the significance of heightened peroxide exposure during continuous high intensity exercise and the mechanisms of PRDX-regulatory control.",
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AU - Wadley, A. J.

AU - Chen, Y. W.

AU - Bennett, S. J.

AU - Lip, G. Y. H.

AU - Turner, J. E.

AU - Fisher, J. P.

AU - Aldred, S.

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N2 - Abstract Introduction: Peroxiredoxin (PRDX) and Thioredoxin (TRX) are antioxidant proteins that control cellular signalling and redox balance, although their response to exercise is unknown. This study aimed to assess key aspects of the PRDX-TRX redox cycle in response to three different modes of exercise. Methods: Healthy males (n=10, mean ± SD: age 22 ± 3 yrs) undertook three exercise trials on separate days: two steady-state cycling trials at a moderate (60% MAX; 27 min, MOD) and high (80% MAX; 20 min, HIGH) intensity, and a low volume high intensity interval training trial (10×1 min 90% MAX, LV-HIIT). Peripheral blood mononuclear cells (PBMCs) were assessed for TRX-1 and over-oxidised PRDX (isoforms I-IV) protein expression before, during and 30 minutes following exercise (post+30). The activities of TRX reductase (TRX-R) and the NF-κB p65 subunit were also assessed. Results: TRX-1 increased during exercise in all trials (MOD +84.5%; HIGH +64.1%; LV-HIIT +205.7%; p<.05), whereas over-oxidised PRDX increased during HIGH only (MOD -28.7%; HIGH +202.9%; LV-HIIT -22.7%; p<.05). TRX-R and NF-κB p65 activity increased during exercise in all trials, with the greatest response in TRX-R activity seen in HIGH (p<.05). Discussion: All trials stimulated a transient increase in TRX-1 protein expression during exercise. Only HIGH induced a transient over-oxidation of PRDX, alongside the greatest change in TRX-R activity. Future studies are needed to clarify the significance of heightened peroxide exposure during continuous high intensity exercise and the mechanisms of PRDX-regulatory control.

AB - Abstract Introduction: Peroxiredoxin (PRDX) and Thioredoxin (TRX) are antioxidant proteins that control cellular signalling and redox balance, although their response to exercise is unknown. This study aimed to assess key aspects of the PRDX-TRX redox cycle in response to three different modes of exercise. Methods: Healthy males (n=10, mean ± SD: age 22 ± 3 yrs) undertook three exercise trials on separate days: two steady-state cycling trials at a moderate (60% MAX; 27 min, MOD) and high (80% MAX; 20 min, HIGH) intensity, and a low volume high intensity interval training trial (10×1 min 90% MAX, LV-HIIT). Peripheral blood mononuclear cells (PBMCs) were assessed for TRX-1 and over-oxidised PRDX (isoforms I-IV) protein expression before, during and 30 minutes following exercise (post+30). The activities of TRX reductase (TRX-R) and the NF-κB p65 subunit were also assessed. Results: TRX-1 increased during exercise in all trials (MOD +84.5%; HIGH +64.1%; LV-HIIT +205.7%; p<.05), whereas over-oxidised PRDX increased during HIGH only (MOD -28.7%; HIGH +202.9%; LV-HIIT -22.7%; p<.05). TRX-R and NF-κB p65 activity increased during exercise in all trials, with the greatest response in TRX-R activity seen in HIGH (p<.05). Discussion: All trials stimulated a transient increase in TRX-1 protein expression during exercise. Only HIGH induced a transient over-oxidation of PRDX, alongside the greatest change in TRX-R activity. Future studies are needed to clarify the significance of heightened peroxide exposure during continuous high intensity exercise and the mechanisms of PRDX-regulatory control.

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SN - 1071-5762

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