Abstract
Bacteria possess complex and varying cell walls with many surface exposed proteins. Sortases are responsible for the covalent attachment of specific proteins to the peptidoglycan of the cell wall of Gram-positive bacteria. Sortase A of Staphylococcus aureus, which is seen as the archetypal sortase, has been shown to be essential for pathogenesis and has therefore received much attention as a potential target for novel therapeutics. Being widely present in Gram-positive bacteria, it is likely that other Gram-positive pathogens also require sortases for their pathogenesis. Sortases have also been shown to be of significant use in a range of industrial applications. We review current knowledge of the sortase family in terms of their structures, functions and mechanisms and summarize work towards their use as antibacterial targets and microbiological tools.
| Original language | English |
|---|---|
| Pages (from-to) | 2097-2114 |
| Number of pages | 118 |
| Journal | FEBS Journal |
| Volume | 282 |
| Issue number | 11 |
| Early online date | 24 Apr 2015 |
| DOIs | |
| Publication status | Published - Jun 2015 |
Fingerprint
Keywords
- Crystal structure
- Inhibitor design
- Molecular mechanism
- Peptide recognition
- Sortase
Cite this
Molecular features of the sortase enzyme family. / Bradshaw, William J.; Davies, Abigail H.; Chambers, Christopher J.; Roberts, April K.; Shone, Clifford C.; Acharya, K. Ravi.
In: FEBS Journal, Vol. 282, No. 11, 06.2015, p. 2097-2114.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular features of the sortase enzyme family
AU - Bradshaw, William J.
AU - Davies, Abigail H.
AU - Chambers, Christopher J.
AU - Roberts, April K.
AU - Shone, Clifford C.
AU - Acharya, K. Ravi
PY - 2015/6
Y1 - 2015/6
N2 - Bacteria possess complex and varying cell walls with many surface exposed proteins. Sortases are responsible for the covalent attachment of specific proteins to the peptidoglycan of the cell wall of Gram-positive bacteria. Sortase A of Staphylococcus aureus, which is seen as the archetypal sortase, has been shown to be essential for pathogenesis and has therefore received much attention as a potential target for novel therapeutics. Being widely present in Gram-positive bacteria, it is likely that other Gram-positive pathogens also require sortases for their pathogenesis. Sortases have also been shown to be of significant use in a range of industrial applications. We review current knowledge of the sortase family in terms of their structures, functions and mechanisms and summarize work towards their use as antibacterial targets and microbiological tools.
AB - Bacteria possess complex and varying cell walls with many surface exposed proteins. Sortases are responsible for the covalent attachment of specific proteins to the peptidoglycan of the cell wall of Gram-positive bacteria. Sortase A of Staphylococcus aureus, which is seen as the archetypal sortase, has been shown to be essential for pathogenesis and has therefore received much attention as a potential target for novel therapeutics. Being widely present in Gram-positive bacteria, it is likely that other Gram-positive pathogens also require sortases for their pathogenesis. Sortases have also been shown to be of significant use in a range of industrial applications. We review current knowledge of the sortase family in terms of their structures, functions and mechanisms and summarize work towards their use as antibacterial targets and microbiological tools.
KW - Crystal structure
KW - Inhibitor design
KW - Molecular mechanism
KW - Peptide recognition
KW - Sortase
UR - http://www.scopus.com/inward/record.url?scp=84928335543&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1111/febs.13288
U2 - 10.1111/febs.13288
DO - 10.1111/febs.13288
M3 - Article
VL - 282
SP - 2097
EP - 2114
JO - FEBS Journal
JF - FEBS Journal
SN - 1742-464X
IS - 11
ER -