Molecular cloning and phylogenetic analysis of a cDNA encoding the cat (Felis domesticus) Ig epsilon constant region

E R Weber, C R Helps, A P Foster, Anthony C F Perry, T J Gruffydd-Jones, L Hall, D A Harbour, W P H Duffus

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A feline splenic cDNA library was screened with a P-32-labelled cDNA probe encoding the canine IgE epsilon heavy chain subunit. A cDNA sequence of 1614 nucleotides encoding the complete feline IgE heavy chain, as well as a portion of a variable region, was identified. A search of the GenBank database revealed an identity of 82% at the nucleotide level and 76% at the amino acid level between the feline epsilon heavy chain sequence and the canine homologue. In a separate study, feline genomic DNA, isolated from whole feline embryo cells, was subjected to PCR amplification using primers based on known partial genomic DNA sequences for the feline CE gene. Following removal of an intron from the 683 bp PCR product, the coding sequence yielded an ORF of 506 bp. The DNA sequence of this PCR clone differed by a single nucleotide from the cDNA clone. This difference is silent, and therefore the proteins encoded by the two sequences are identical over the regions cloned and sequenced. Phylogenetic analysis of the constant regions of nine immunoglobulin epsilon genes revealed that the feline cDNA is most similar to the canine homologue.
Original languageEnglish
Pages (from-to)299-308
Number of pages10
JournalVeterinary Immunology and Immunopathology
Volume76
Issue number3-4
Publication statusPublished - 2000

Fingerprint

Immunoglobulin Constant Regions
Felis
Felidae
Molecular Cloning
molecular cloning
Cats
Complementary DNA
cats
phylogeny
Canidae
nucleotide sequences
Polymerase Chain Reaction
Immunoglobulin E
dogs
Nucleotides
Clone Cells
nucleotides
clones
genomics
Immunoglobulin Genes

Cite this

Weber, E. R., Helps, C. R., Foster, A. P., Perry, A. C. F., Gruffydd-Jones, T. J., Hall, L., ... Duffus, W. P. H. (2000). Molecular cloning and phylogenetic analysis of a cDNA encoding the cat (Felis domesticus) Ig epsilon constant region. Veterinary Immunology and Immunopathology, 76(3-4), 299-308.

Molecular cloning and phylogenetic analysis of a cDNA encoding the cat (Felis domesticus) Ig epsilon constant region. / Weber, E R; Helps, C R; Foster, A P; Perry, Anthony C F; Gruffydd-Jones, T J; Hall, L; Harbour, D A; Duffus, W P H.

In: Veterinary Immunology and Immunopathology, Vol. 76, No. 3-4, 2000, p. 299-308.

Research output: Contribution to journalArticle

Weber, ER, Helps, CR, Foster, AP, Perry, ACF, Gruffydd-Jones, TJ, Hall, L, Harbour, DA & Duffus, WPH 2000, 'Molecular cloning and phylogenetic analysis of a cDNA encoding the cat (Felis domesticus) Ig epsilon constant region', Veterinary Immunology and Immunopathology, vol. 76, no. 3-4, pp. 299-308.
Weber, E R ; Helps, C R ; Foster, A P ; Perry, Anthony C F ; Gruffydd-Jones, T J ; Hall, L ; Harbour, D A ; Duffus, W P H. / Molecular cloning and phylogenetic analysis of a cDNA encoding the cat (Felis domesticus) Ig epsilon constant region. In: Veterinary Immunology and Immunopathology. 2000 ; Vol. 76, No. 3-4. pp. 299-308.
@article{c9547c5307f94d6a85d9be6fdf47e3e3,
title = "Molecular cloning and phylogenetic analysis of a cDNA encoding the cat (Felis domesticus) Ig epsilon constant region",
abstract = "A feline splenic cDNA library was screened with a P-32-labelled cDNA probe encoding the canine IgE epsilon heavy chain subunit. A cDNA sequence of 1614 nucleotides encoding the complete feline IgE heavy chain, as well as a portion of a variable region, was identified. A search of the GenBank database revealed an identity of 82{\%} at the nucleotide level and 76{\%} at the amino acid level between the feline epsilon heavy chain sequence and the canine homologue. In a separate study, feline genomic DNA, isolated from whole feline embryo cells, was subjected to PCR amplification using primers based on known partial genomic DNA sequences for the feline CE gene. Following removal of an intron from the 683 bp PCR product, the coding sequence yielded an ORF of 506 bp. The DNA sequence of this PCR clone differed by a single nucleotide from the cDNA clone. This difference is silent, and therefore the proteins encoded by the two sequences are identical over the regions cloned and sequenced. Phylogenetic analysis of the constant regions of nine immunoglobulin epsilon genes revealed that the feline cDNA is most similar to the canine homologue.",
author = "Weber, {E R} and Helps, {C R} and Foster, {A P} and Perry, {Anthony C F} and Gruffydd-Jones, {T J} and L Hall and Harbour, {D A} and Duffus, {W P H}",
year = "2000",
language = "English",
volume = "76",
pages = "299--308",
journal = "Veterinary Immunology and Immunopathology",
issn = "0165-2427",
publisher = "Elsevier",
number = "3-4",

}

TY - JOUR

T1 - Molecular cloning and phylogenetic analysis of a cDNA encoding the cat (Felis domesticus) Ig epsilon constant region

AU - Weber, E R

AU - Helps, C R

AU - Foster, A P

AU - Perry, Anthony C F

AU - Gruffydd-Jones, T J

AU - Hall, L

AU - Harbour, D A

AU - Duffus, W P H

PY - 2000

Y1 - 2000

N2 - A feline splenic cDNA library was screened with a P-32-labelled cDNA probe encoding the canine IgE epsilon heavy chain subunit. A cDNA sequence of 1614 nucleotides encoding the complete feline IgE heavy chain, as well as a portion of a variable region, was identified. A search of the GenBank database revealed an identity of 82% at the nucleotide level and 76% at the amino acid level between the feline epsilon heavy chain sequence and the canine homologue. In a separate study, feline genomic DNA, isolated from whole feline embryo cells, was subjected to PCR amplification using primers based on known partial genomic DNA sequences for the feline CE gene. Following removal of an intron from the 683 bp PCR product, the coding sequence yielded an ORF of 506 bp. The DNA sequence of this PCR clone differed by a single nucleotide from the cDNA clone. This difference is silent, and therefore the proteins encoded by the two sequences are identical over the regions cloned and sequenced. Phylogenetic analysis of the constant regions of nine immunoglobulin epsilon genes revealed that the feline cDNA is most similar to the canine homologue.

AB - A feline splenic cDNA library was screened with a P-32-labelled cDNA probe encoding the canine IgE epsilon heavy chain subunit. A cDNA sequence of 1614 nucleotides encoding the complete feline IgE heavy chain, as well as a portion of a variable region, was identified. A search of the GenBank database revealed an identity of 82% at the nucleotide level and 76% at the amino acid level between the feline epsilon heavy chain sequence and the canine homologue. In a separate study, feline genomic DNA, isolated from whole feline embryo cells, was subjected to PCR amplification using primers based on known partial genomic DNA sequences for the feline CE gene. Following removal of an intron from the 683 bp PCR product, the coding sequence yielded an ORF of 506 bp. The DNA sequence of this PCR clone differed by a single nucleotide from the cDNA clone. This difference is silent, and therefore the proteins encoded by the two sequences are identical over the regions cloned and sequenced. Phylogenetic analysis of the constant regions of nine immunoglobulin epsilon genes revealed that the feline cDNA is most similar to the canine homologue.

M3 - Article

VL - 76

SP - 299

EP - 308

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

IS - 3-4

ER -