Modular development of a prototype point of care molecular diagnostic platform for sexually transmitted infections

Manoharanehru Branavan, Ruth E Mackay, Pascal Craw, Angel Naveenathayalan, Jeremy C Ahern, Tulasi Sivanesan, Chris Hudson, Thomas Stead, Jessica Kremer, Neha Garg, Mark Baker, Syed T Sadiq, Wamadeva Balachandran

Research output: Contribution to journalArticlepeer-review

13 Citations (SciVal)


This paper presents the design of a modular point of care test platform that integrates a proprietary sample collection device directly with a microfluidic cartridge. Cell lysis, within the cartridge, is conducted using a chemical method and nucleic acid purification is done on an activated cellulose membrane. The microfluidic device incorporates passive mixing of the lysis-binding buffers and sample using a serpentine channel. Results have shown extraction efficiencies for this new membrane of 69% and 57% compared to the commercial Qiagen extraction method of 85% and 59.4% for 0.1ng/µL and 100ng/µL salmon sperm DNA respectively spiked in phosphate buffered solution. Extraction experiments using the serpentine passive mixer cartridges incorporating lysis and nucleic acid purification showed extraction efficiency around 80% of the commercial Qiagen kit. Isothermal amplification was conducted using thermophillic helicase dependant amplification and recombinase polymerase amplification. A low cost benchtop real-time isothermal amplification platform has been developed capable of running six amplifications simultaneously. Results show that the platform is capable of detecting 1.32×10(6) of sample DNA through thermophillic helicase dependant amplification and 1×10(5) copy numbers Chlamydia trachomatis genomic DNA within 10min through recombinase polymerase nucleic acid amplification tests.

Original languageEnglish
Pages (from-to)741-8
Number of pages8
JournalMedical Engineering & Physics
Issue number8
Publication statusPublished - 22 Apr 2016

Bibliographical note

Copyright © 2016 The Author(s). Published by Elsevier Ltd.. All rights reserved.


  • Animals
  • DNA/genetics
  • Hot Temperature
  • Lab-On-A-Chip Devices
  • Molecular Diagnostic Techniques/instrumentation
  • Nucleic Acid Amplification Techniques
  • Point-of-Care Systems
  • Sexually Transmitted Diseases/diagnosis


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