Modified Filamentous Bacteriophage as a Scaffold for Carbon Nanofiber

Katarzyna Szot-Karpińska, Piotr Golec, Adam Leśniewski, Barbara Pałys, Frank Marken, Joanna Niedziółka-Jönsson, Grzegorz Wȩgrzyn, Marcin Łoś

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5 Citations (Scopus)

Abstract

With the advent of nanotechnology, carbon nanomaterials such as carbon nanofibers (CNF) have aroused substantial interest in various research fields, including energy storage and sensing. Further improvement of their properties might be achieved via the application of viral particles such as bacteriophages. In this report, we present a filamentous M13 bacteriophage with a point mutation in gene VII (pVII-mutant-M13) that selectively binds to the carbon nanofibers to form 3D structures. The phage-display technique was utilized for the selection of the pVII-mutant-M13 phage from the phage display peptide library. The properties of this phage make it a prospective candidate for a scaffold material for CNFs. The results for binding of CNF by mutant phage were compared with those for maternal bacteriophage (pVII-M13). The efficiency of binding between pVII-mutant-M13 and CNF is about 2 orders of magnitude higher compared to that of the pVII-M13. Binding affinity between pVII-mutant-M13 and CNF was also characterized using atomic force microscopy, scanning electron microscopy, and transmission electron microscopy, which confirmed the specificity of the interaction of the phage pVII-mutant-M13 and the CNF; the binding occurs via the phage's ending, where the mutated pVII protein is located. No similar behavior has been observed for other carbon nanomaterials such as graphite, reduced graphene oxide, single-walled carbon nanotubes, and multiwalled carbon nanotubes. Infrared spectra confirmed differences in the interaction with CNF between the pVII-mutant-M13 and the pVII-M13. Basing on conducted research, we hypothesize that the interactions are noncovalent in nature, with π-π interactions playing the dominant role. Herein, the new bioconjugate material is introduced.

Original languageEnglish
Pages (from-to)2900-2910
Number of pages11
JournalBioconjugate Chemistry
Volume27
Issue number12
Early online date16 Nov 2016
DOIs
Publication statusPublished - 21 Dec 2016

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Szot-Karpińska, K., Golec, P., Leśniewski, A., Pałys, B., Marken, F., Niedziółka-Jönsson, J., Wȩgrzyn, G., & Łoś, M. (2016). Modified Filamentous Bacteriophage as a Scaffold for Carbon Nanofiber. Bioconjugate Chemistry, 27(12), 2900-2910. https://doi.org/10.1021/acs.bioconjchem.6b00555