Mitochondrial Respiration and Oxygen Tension

Daniel S Shaw, Karlia Meitha, Michael J Considine, Christine H Foyer

Research output: Contribution to journalArticle

Abstract

Measurements of respiration and oxygen tension in plant organs allow a precise understanding of mitochondrial capacity and function within the context of cellular oxygen metabolism. Here we describe methods that can be routinely used for the isolation of intact mitochondria, and the determination of respiratory electron transport, together with techniques for in vivo determination of oxygen tension and measurement of respiration by both CO2 production and O2 consumption that enables calculation of the respiratory quotient [CO2]/[O2].

Original languageEnglish
Article number11
Pages (from-to)97-113
Number of pages17
JournalMethods in molecular biology (Clifton, N.J.)
Volume1670
Early online date5 Sep 2017
DOIs
Publication statusPublished - 2017

Keywords

  • Carbon Dioxide/metabolism
  • Cell Fractionation/methods
  • Cell Respiration
  • Centrifugation, Density Gradient
  • Electron Transport
  • Mitochondria/metabolism
  • Oxygen/metabolism
  • Oxygen Consumption
  • Partial Pressure

Cite this

Shaw, D. S., Meitha, K., Considine, M. J., & Foyer, C. H. (2017). Mitochondrial Respiration and Oxygen Tension. Methods in molecular biology (Clifton, N.J.), 1670, 97-113. [11]. https://doi.org/10.1007/978-1-4939-7292-0_11