Microinjection of medaka embryos for use as a model genetic organism

Sean R Porazinski, Huijia Wang, Makoto Furutani-Seiki

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

In this video, we demonstrate the technique of microinjection into one-cell stage medaka embryos. Medaka is a small egg-laying freshwater fish that allows both genetic and embryological analyses and is one of the vertebrate model organisms in which genome-wide phenotype-driven mutant screens were carried out 1, as in zebrafish and the mouse. Divergence of functional overlap of related genes between medaka and zebrafish allows identification of novel phenotypes that are unidentifiable in a single species 2, thus medaka and zebrafish are complementary for genetic dissection of vertebrate genome functions. To take advantage of medaka fish whose embryos are transparent and develop externally, microinjection is an essential technique to inject cell-tracers for labeling cells, mRNAs or anti-sense oligonucleotides for over-expressing and knocking-down genes of interest, and DNAs for making transgenic lines.
Original languageEnglish
Article numbere1937
Number of pages4
JournalJournal of Visualized Experiments
Volume2010
Issue number46
DOIs
Publication statusPublished - 22 Dec 2010

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