Abstract

Organoids are three-dimensional multicellular structures capable of mimicking tissue structure and tumour heterogeneity better than other available biological model systems. They are a powerful new enabling technology in drug discovery because they can give more accurate test results while enabling high throughput screening of drug candidates. However, traditional manual culture and expansion of organoids is labour intensive and expensive. In order for organoids to be widely used in industrial and clinical applications, their robust production must be scaled up efficiently. Cellesce Ldt has developed a process to grow large numbers of organoids for commercial drug screening but the methods rely heavily on very expensive animal-derived matrix scaffolds for organoid encapsulation, mainly Matrigel. These materials are both expensive to produce and are prone to batch-to-batch variations in the bioactive molecules which they contain. In this research, non-animal derived polymers were investigated to reduce Matrigel use as 3D organoid matrix, focusing on the production of cell-containing hydrogel microbeads. Material A and B, both derived hydrogel-forming biomaterials, were selected to form blends with Matrigel, thus providing a more sustainable and cost-effective means of organoid generation. The microbeads were produced by electrospraying and compared in terms of their physical properties. Finally, the ability of the cells to grow and form organoids within these blends was assessed and compared with the current method using Matrigel. This study concluded that the material A-based blends were the only ones that supported organoid growth, with the material A being the optimum blend among the others. The main outcome of this research will be the basis for future bioreactor and scale-up studies.
Original languageEnglish
Publication statusPublished - 21 May 2018
EventAdvances in Cell and Tissue Culture Conference - The School of Biosciences at Cardiff University, Cardiff, UK United Kingdom
Duration: 21 May 201823 May 2018
Conference number: 10th
http://actc2018.com/

Conference

ConferenceAdvances in Cell and Tissue Culture Conference
Abbreviated titleACTC 2018
CountryUK United Kingdom
CityCardiff
Period21/05/1823/05/18
Internet address

Fingerprint

Hydrogels
Screening
Scaffolds (biology)
Bioreactors
Encapsulation
Biomaterials
Tumors
Animals
Physical properties
Throughput
Personnel
Tissue
Molecules
Polymers
Costs
Drug Discovery

Cite this

Pinheiro De Lucena-Thomas, J., Luetchford, K., Ellis, M., & De Bank, P. (2018). Microgel Matrices for Human Cancer Organoid Production. Poster session presented at Advances in Cell and Tissue Culture Conference, Cardiff, UK United Kingdom.

Microgel Matrices for Human Cancer Organoid Production. / Pinheiro De Lucena-Thomas, Jessica; Luetchford, Kimberley; Ellis, Marianne; De Bank, Paul.

2018. Poster session presented at Advances in Cell and Tissue Culture Conference, Cardiff, UK United Kingdom.

Research output: Contribution to conferencePoster

Pinheiro De Lucena-Thomas, J, Luetchford, K, Ellis, M & De Bank, P 2018, 'Microgel Matrices for Human Cancer Organoid Production' Advances in Cell and Tissue Culture Conference, Cardiff, UK United Kingdom, 21/05/18 - 23/05/18, .
Pinheiro De Lucena-Thomas J, Luetchford K, Ellis M, De Bank P. Microgel Matrices for Human Cancer Organoid Production. 2018. Poster session presented at Advances in Cell and Tissue Culture Conference, Cardiff, UK United Kingdom.
Pinheiro De Lucena-Thomas, Jessica ; Luetchford, Kimberley ; Ellis, Marianne ; De Bank, Paul. / Microgel Matrices for Human Cancer Organoid Production. Poster session presented at Advances in Cell and Tissue Culture Conference, Cardiff, UK United Kingdom.
@conference{46492a8f09ca469b9bf0999421717a4a,
title = "Microgel Matrices for Human Cancer Organoid Production",
abstract = "Organoids are three-dimensional multicellular structures capable of mimicking tissue structure and tumour heterogeneity better than other available biological model systems. They are a powerful new enabling technology in drug discovery because they can give more accurate test results while enabling high throughput screening of drug candidates. However, traditional manual culture and expansion of organoids is labour intensive and expensive. In order for organoids to be widely used in industrial and clinical applications, their robust production must be scaled up efficiently. Cellesce Ldt has developed a process to grow large numbers of organoids for commercial drug screening but the methods rely heavily on very expensive animal-derived matrix scaffolds for organoid encapsulation, mainly Matrigel. These materials are both expensive to produce and are prone to batch-to-batch variations in the bioactive molecules which they contain. In this research, non-animal derived polymers were investigated to reduce Matrigel use as 3D organoid matrix, focusing on the production of cell-containing hydrogel microbeads. Material A and B, both derived hydrogel-forming biomaterials, were selected to form blends with Matrigel, thus providing a more sustainable and cost-effective means of organoid generation. The microbeads were produced by electrospraying and compared in terms of their physical properties. Finally, the ability of the cells to grow and form organoids within these blends was assessed and compared with the current method using Matrigel. This study concluded that the material A-based blends were the only ones that supported organoid growth, with the material A being the optimum blend among the others. The main outcome of this research will be the basis for future bioreactor and scale-up studies.",
author = "{Pinheiro De Lucena-Thomas}, Jessica and Kimberley Luetchford and Marianne Ellis and {De Bank}, Paul",
year = "2018",
month = "5",
day = "21",
language = "English",
note = "Advances in Cell and Tissue Culture Conference, ACTC 2018 ; Conference date: 21-05-2018 Through 23-05-2018",
url = "http://actc2018.com/",

}

TY - CONF

T1 - Microgel Matrices for Human Cancer Organoid Production

AU - Pinheiro De Lucena-Thomas, Jessica

AU - Luetchford, Kimberley

AU - Ellis, Marianne

AU - De Bank, Paul

PY - 2018/5/21

Y1 - 2018/5/21

N2 - Organoids are three-dimensional multicellular structures capable of mimicking tissue structure and tumour heterogeneity better than other available biological model systems. They are a powerful new enabling technology in drug discovery because they can give more accurate test results while enabling high throughput screening of drug candidates. However, traditional manual culture and expansion of organoids is labour intensive and expensive. In order for organoids to be widely used in industrial and clinical applications, their robust production must be scaled up efficiently. Cellesce Ldt has developed a process to grow large numbers of organoids for commercial drug screening but the methods rely heavily on very expensive animal-derived matrix scaffolds for organoid encapsulation, mainly Matrigel. These materials are both expensive to produce and are prone to batch-to-batch variations in the bioactive molecules which they contain. In this research, non-animal derived polymers were investigated to reduce Matrigel use as 3D organoid matrix, focusing on the production of cell-containing hydrogel microbeads. Material A and B, both derived hydrogel-forming biomaterials, were selected to form blends with Matrigel, thus providing a more sustainable and cost-effective means of organoid generation. The microbeads were produced by electrospraying and compared in terms of their physical properties. Finally, the ability of the cells to grow and form organoids within these blends was assessed and compared with the current method using Matrigel. This study concluded that the material A-based blends were the only ones that supported organoid growth, with the material A being the optimum blend among the others. The main outcome of this research will be the basis for future bioreactor and scale-up studies.

AB - Organoids are three-dimensional multicellular structures capable of mimicking tissue structure and tumour heterogeneity better than other available biological model systems. They are a powerful new enabling technology in drug discovery because they can give more accurate test results while enabling high throughput screening of drug candidates. However, traditional manual culture and expansion of organoids is labour intensive and expensive. In order for organoids to be widely used in industrial and clinical applications, their robust production must be scaled up efficiently. Cellesce Ldt has developed a process to grow large numbers of organoids for commercial drug screening but the methods rely heavily on very expensive animal-derived matrix scaffolds for organoid encapsulation, mainly Matrigel. These materials are both expensive to produce and are prone to batch-to-batch variations in the bioactive molecules which they contain. In this research, non-animal derived polymers were investigated to reduce Matrigel use as 3D organoid matrix, focusing on the production of cell-containing hydrogel microbeads. Material A and B, both derived hydrogel-forming biomaterials, were selected to form blends with Matrigel, thus providing a more sustainable and cost-effective means of organoid generation. The microbeads were produced by electrospraying and compared in terms of their physical properties. Finally, the ability of the cells to grow and form organoids within these blends was assessed and compared with the current method using Matrigel. This study concluded that the material A-based blends were the only ones that supported organoid growth, with the material A being the optimum blend among the others. The main outcome of this research will be the basis for future bioreactor and scale-up studies.

M3 - Poster

ER -