Microfluidic assays without sample preparation: New microfluidic strategies for overcoming biological matrix interference

A. I. Barbosa, N. M. Reis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We explore a novel microfluidic approach for assays without sample preparation which can revolutionize point-of-care diagnostics industry due to its simplicity and affordability. This studies aims at understanding the role of surface coverage and diffusion distance in antibody-antigen equilibrium in Teflon-FEP microcapillaries. IL-1β human cytokine sandwich assay showed higher surface coverages in ∼200 μm diameter microcapillaries eliminating biological matrix effect interference, being highly desirable for rapid point-of-care (POC) tests.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Place of PublicationDublin, Ireland
PublisherChemical and Biological Microsystems Society
Pages1238-1239
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 1 Jan 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period9/10/1613/10/16

Keywords

  • Diagnostics
  • Matrix effect
  • Microcapillaries
  • Surface coverage
  • Teflon-FEP

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Barbosa, A. I., & Reis, N. M. (2016). Microfluidic assays without sample preparation: New microfluidic strategies for overcoming biological matrix interference. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 1238-1239). Chemical and Biological Microsystems Society.