Microcin J25 induces the opening of the mitochondrial transition pore and cytochrome c release through superoxide generation

María Niklison Chirou, Augusto Bellomio, Fernando Dupuy, Beatriz Arcuri, Carlos Minahk, Roberto Morero

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Microcin J25, an antimicrobial lasso-structure peptide, induces the opening of mitochondrial permeability transition pores and the subsequent loss of cytochrome c. The microcin J25 effect is mediated by the stimulation of superoxide anion overproduction. An increased uptake of calcium is also involved in this process. Additional studies with superoxide dismutase, ascorbic acid and different specific inhibitors, such as ruthenium red, cyclosporin A and Mn2+, allowed us to establish a time sequence of events starting with the binding of microcin J25, followed by superoxide anion overproduction, opening of mitochondrial permeability transition pores, mitochondrial swelling and the concomitant leakage of cytochrome c.

Original languageEnglish
Pages (from-to)4088-4096
Number of pages9
JournalFEBS Journal
Volume275
Issue number16
DOIs
Publication statusPublished - 1 Aug 2008

Keywords

  • Antibiotics
  • Ca
  • Cytochrome c
  • Microcin
  • Mitochondria

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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