Microcin J25-Ga induces apoptosis in mammalian cells by inhibiting mitochondrial RNA-polymerase

María V. Niklison-Chirou; Fernando Dupuy; Lucila Saavedra; Elvira Hebert; Claudia Banchio; Carlos Minahk; Roberto D. Morero

doi:10.1016/j.peptides.2011.01.003

Microcin J25-Ga induces apoptosis in mammalian cells by inhibiting mitochondrial RNA-polymerase

María V. Niklison-Chirou
, Fernando Dupuy
, Lucila Saavedra
, Elvira Hebert
, Claudia Banchio
, Carlos Minahk
, Roberto D. Morero

Department of Life Sciences

Instituto de Química Biológica Dr. Bernabe Bloj
Centro de Referencia para Lactobacilos (CERELA-CONICET)
Universidad Nacional de Rosario

Research output: Contribution to journal › Article › peer-review

11 Citations (SciVal)

Abstract

MccJ25, an antimicrobial peptide, was unable to cause apoptosis of COS-7 cells in spite of inducing reactive-oxygen species overproduction as well as cytochrome c release from isolated mitochondria. Surprisingly, MccJ25-Ga, an amidated variant of MccJ25 that displays similar anti-mitochondrial effects, did induce apoptosis in COS-7. The only difference found between the activities of these peptides was the unpredicted inhibition of mitochondrial RNA synthesis by MccJ25-Ga. These results led us to hypothesize that both mitochondrial RNA polymerase and mitochondrial membrane might be the molecular targets of MccJ25-Ga in mitochondria and this combined effect may lead to apoptosis.

Original language	English
Pages (from-to)	832-834
Number of pages	3
Journal	Peptides
Volume	32
Issue number	4
DOIs	https://doi.org/10.1016/j.peptides.2011.01.003
Publication status	Published - Apr 2011

Funding

Financial support was provided by CONICET (Grant PIP 4996 ) and CIUNT (Grant 26/D228 ) and the Agencia Nacional de Promoción Científica y Técnica (PICTO 843, PAE 22642). M.V.N. and F.D. were recipient of a CONICET fellowship. R.D.M., C.M., L.S., E.H. and C.B. are career investigator of CONICET. We want to thank to Dr. Manuel Aybar and Dr. Tristán Agüero for their generous assistance.

Keywords

Apoptosis
Microcin
Mitochondria
RNA-polymerase

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/j.peptides.2011.01.003

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title = "Microcin J25-Ga induces apoptosis in mammalian cells by inhibiting mitochondrial RNA-polymerase",

abstract = "MccJ25, an antimicrobial peptide, was unable to cause apoptosis of COS-7 cells in spite of inducing reactive-oxygen species overproduction as well as cytochrome c release from isolated mitochondria. Surprisingly, MccJ25-Ga, an amidated variant of MccJ25 that displays similar anti-mitochondrial effects, did induce apoptosis in COS-7. The only difference found between the activities of these peptides was the unpredicted inhibition of mitochondrial RNA synthesis by MccJ25-Ga. These results led us to hypothesize that both mitochondrial RNA polymerase and mitochondrial membrane might be the molecular targets of MccJ25-Ga in mitochondria and this combined effect may lead to apoptosis.",

keywords = "Apoptosis, Microcin, Mitochondria, RNA-polymerase",

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T1 - Microcin J25-Ga induces apoptosis in mammalian cells by inhibiting mitochondrial RNA-polymerase

AU - Niklison-Chirou, María V.

AU - Dupuy, Fernando

AU - Saavedra, Lucila

AU - Hebert, Elvira

AU - Banchio, Claudia

AU - Minahk, Carlos

AU - Morero, Roberto D.

PY - 2011/4

Y1 - 2011/4

N2 - MccJ25, an antimicrobial peptide, was unable to cause apoptosis of COS-7 cells in spite of inducing reactive-oxygen species overproduction as well as cytochrome c release from isolated mitochondria. Surprisingly, MccJ25-Ga, an amidated variant of MccJ25 that displays similar anti-mitochondrial effects, did induce apoptosis in COS-7. The only difference found between the activities of these peptides was the unpredicted inhibition of mitochondrial RNA synthesis by MccJ25-Ga. These results led us to hypothesize that both mitochondrial RNA polymerase and mitochondrial membrane might be the molecular targets of MccJ25-Ga in mitochondria and this combined effect may lead to apoptosis.

AB - MccJ25, an antimicrobial peptide, was unable to cause apoptosis of COS-7 cells in spite of inducing reactive-oxygen species overproduction as well as cytochrome c release from isolated mitochondria. Surprisingly, MccJ25-Ga, an amidated variant of MccJ25 that displays similar anti-mitochondrial effects, did induce apoptosis in COS-7. The only difference found between the activities of these peptides was the unpredicted inhibition of mitochondrial RNA synthesis by MccJ25-Ga. These results led us to hypothesize that both mitochondrial RNA polymerase and mitochondrial membrane might be the molecular targets of MccJ25-Ga in mitochondria and this combined effect may lead to apoptosis.

KW - Apoptosis

KW - Microcin

KW - Mitochondria

KW - RNA-polymerase

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DO - 10.1016/j.peptides.2011.01.003

M3 - Article

AN - SCOPUS:79952814524

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VL - 32

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JO - Peptides

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ER -

Microcin J25-Ga induces apoptosis in mammalian cells by inhibiting mitochondrial RNA-polymerase

Abstract

Funding

Keywords

ASJC Scopus subject areas

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