Micro-magnetic resonance imaging of avian embryos

Xiaojing Li, Jia Liu, Megan Davey, Suzanne Duce, Neema Jaberi, Gang Liu, Gemma Davidson, Seaneen Tenent, Ruth Mahood, Phoebe Brown, Craig Cunningham, Andrew Bain, Kevin Beattie, Laura Ann Mcdonald, Katy Schmidt, Matthew Towers, Cheryll Tickle, Sandy Chudek

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26 Citations (Scopus)

Abstract

Chick embryos are useful models for probing developmental mechanisms including those involved in organogenesis. In addition to classic embryological manipulations, it is possible to test the function of molecules and genes while the embryo remains within the egg. Here we define conditions for imaging chick embryo anatomy and for visualising living quail embryos. We focus on the developing limb and describe how different tissues can be imaged using micro-magnetic resonance imaging and this information then synthesised, using a three-dimensional visualisation package, into detailed anatomy. We illustrate the potential for micro-magnetic resonance imaging to analyse phenotypic changes following chick limb manipulation. The work with the living quail embryos lays the foundations for using micro-magnetic resonance imaging as an experimental tool to follow the consequences of such manipulations over time.
Original languageEnglish
Pages (from-to)798-809
JournalJournal Of Anatomy
Volume211
Issue number6
DOIs
Publication statusPublished - Dec 2007

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Li, X., Liu, J., Davey, M., Duce, S., Jaberi, N., Liu, G., ... Chudek, S. (2007). Micro-magnetic resonance imaging of avian embryos. Journal Of Anatomy, 211(6), 798-809. https://doi.org/10.1111/j.1469-7580.2007.00825.x