Micro-computed tomography for visualizing limb skeletal regeneration in young Xenopus frogs

Y Chen; G Lin; Y-C Chen; A Fok; J Slack

doi:10.1002/ar.22496

Micro-computed tomography for visualizing limb skeletal regeneration in young Xenopus frogs

Y Chen, G Lin, Y-C Chen, A Fok, J Slack

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (SciVal)

Abstract

For studies of vertebrate limb regeneration it is often desirable to visualize the regenerated skeleton, which is mostly cartilage, and also section the specimen for histological or immunohistochemical visualization of other tissues. However, the normal skeletal staining techniques are incompatible with immunohistochemistry. Here, we describe a contrast-based micro-computed tomography (microCT) method for direct and nondestructive observation of regenerated cartilage spikes in Xenopus frog limbs. In addition, we show that contrast based microCT imaging is compatible with immunohistochemistry protocols. This approach provides versatile and high contrast images of the cartilage allowing us to measure the regenerated skeletal structure in detail as well as carrying out the other types of analysis. It opens a wide range of potential microCT applications in research on vertebrate limb regeneration.

Copyright © 2012 Wiley Periodicals, Inc.

Original language	English
Pages (from-to)	1562-1565
Number of pages	3
Journal	The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology
Volume	295
Issue number	10
DOIs	https://doi.org/10.1002/ar.22496
Publication status	Published - Oct 2012

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abstract = "For studies of vertebrate limb regeneration it is often desirable to visualize the regenerated skeleton, which is mostly cartilage, and also section the specimen for histological or immunohistochemical visualization of other tissues. However, the normal skeletal staining techniques are incompatible with immunohistochemistry. Here, we describe a contrast-based micro-computed tomography (microCT) method for direct and nondestructive observation of regenerated cartilage spikes in Xenopus frog limbs. In addition, we show that contrast based microCT imaging is compatible with immunohistochemistry protocols. This approach provides versatile and high contrast images of the cartilage allowing us to measure the regenerated skeletal structure in detail as well as carrying out the other types of analysis. It opens a wide range of potential microCT applications in research on vertebrate limb regeneration.Copyright {\textcopyright} 2012 Wiley Periodicals, Inc.",

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AU - Fok, A

AU - Slack, J

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AB - For studies of vertebrate limb regeneration it is often desirable to visualize the regenerated skeleton, which is mostly cartilage, and also section the specimen for histological or immunohistochemical visualization of other tissues. However, the normal skeletal staining techniques are incompatible with immunohistochemistry. Here, we describe a contrast-based micro-computed tomography (microCT) method for direct and nondestructive observation of regenerated cartilage spikes in Xenopus frog limbs. In addition, we show that contrast based microCT imaging is compatible with immunohistochemistry protocols. This approach provides versatile and high contrast images of the cartilage allowing us to measure the regenerated skeletal structure in detail as well as carrying out the other types of analysis. It opens a wide range of potential microCT applications in research on vertebrate limb regeneration.Copyright © 2012 Wiley Periodicals, Inc.

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JO - The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology

JF - The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology

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ER -

Micro-computed tomography for visualizing limb skeletal regeneration in young Xenopus frogs

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