Micro-computed tomography for visualizing limb skeletal regeneration in young Xenopus frogs

Y Chen, G Lin, Y-C Chen, A Fok, J Slack

Research output: Contribution to journalArticle

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Abstract

For studies of vertebrate limb regeneration it is often desirable to visualize the regenerated skeleton, which is mostly cartilage, and also section the specimen for histological or immunohistochemical visualization of other tissues. However, the normal skeletal staining techniques are incompatible with immunohistochemistry. Here, we describe a contrast-based micro-computed tomography (microCT) method for direct and nondestructive observation of regenerated cartilage spikes in Xenopus frog limbs. In addition, we show that contrast based microCT imaging is compatible with immunohistochemistry protocols. This approach provides versatile and high contrast images of the cartilage allowing us to measure the regenerated skeletal structure in detail as well as carrying out the other types of analysis. It opens a wide range of potential microCT applications in research on vertebrate limb regeneration.

Copyright © 2012 Wiley Periodicals, Inc.
LanguageEnglish
Pages1562-1565
Number of pages3
JournalThe Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology
Volume295
Issue number10
DOIs
StatusPublished - Oct 2012

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micro-computed tomography
Xenopus
limbs (animal)
cartilage
frog
Anura
tomography
Cartilage
limb
frogs
Regeneration
Extremities
regeneration
Tomography
immunohistochemistry
Vertebrates
vertebrate
Immunohistochemistry
vertebrates
Skeleton

Cite this

Micro-computed tomography for visualizing limb skeletal regeneration in young Xenopus frogs. / Chen, Y; Lin, G; Chen, Y-C; Fok, A; Slack, J.

In: The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology, Vol. 295, No. 10, 10.2012, p. 1562-1565.

Research output: Contribution to journalArticle

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