METHOD OF DETECTING PEROXYNITRITE USING A COMPLEX OF A SACCHARIDE AND AN ARYLBORONATE-BASED FLUORESCENT PROBE

Tony James (Inventor), Steven Bull (Inventor), Xiaolong Sun (Inventor)

Research output: Patent

Abstract

A method of detecting peroxynitrite in a sample is described comprising the steps of: (a) providing a complex of a saccharide with an aryl boronate compound of formula (I): Fp - L1 - Z - L2 - Ar - B(OH)2 (I) wherein: Fp comprises a fluorophore; L1 and L2 are linker groups; Z is a fluorescence switch; and Ar is optionally substituted aryl; (b) contacting said aryl boronate- saccharide complex with said sample, whereby peroxynitrite in said sample cleaves said aryl boronate- saccharide complex to produce a compound of formula (II): F - L1 - Z - L2 - Ar - OH (II); and (c) detecting a decrease in a fluorescence intensity of said fluorophore resulting from said cleavage reaction in step (b). Peroxynitrite reacts quantitatively, rapidly, and selectively in step (b) of the reaction, whereby medical conditions associated with elevated peroxynitrite can be diagnosed. Also provided are compounds of formula (I) for use in the methods.
Original languageEnglish
Patent numberWO 2015/128622 (EP3110827, US2017059573)
Priority date27/02/14
Filing date24/02/15
Publication statusPublished - 2019

Cite this

METHOD OF DETECTING PEROXYNITRITE USING A COMPLEX OF A SACCHARIDE AND AN ARYLBORONATE-BASED FLUORESCENT PROBE. / James, Tony (Inventor); Bull, Steven (Inventor); Sun, Xiaolong (Inventor).

Patent No.: WO 2015/128622 (EP3110827, US2017059573). Feb 24, 2015.

Research output: Patent

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title = "METHOD OF DETECTING PEROXYNITRITE USING A COMPLEX OF A SACCHARIDE AND AN ARYLBORONATE-BASED FLUORESCENT PROBE",
abstract = "A method of detecting peroxynitrite in a sample is described comprising the steps of: (a) providing a complex of a saccharide with an aryl boronate compound of formula (I): Fp - L1 - Z - L2 - Ar - B(OH)2 (I) wherein: Fp comprises a fluorophore; L1 and L2 are linker groups; Z is a fluorescence switch; and Ar is optionally substituted aryl; (b) contacting said aryl boronate- saccharide complex with said sample, whereby peroxynitrite in said sample cleaves said aryl boronate- saccharide complex to produce a compound of formula (II): F - L1 - Z - L2 - Ar - OH (II); and (c) detecting a decrease in a fluorescence intensity of said fluorophore resulting from said cleavage reaction in step (b). Peroxynitrite reacts quantitatively, rapidly, and selectively in step (b) of the reaction, whereby medical conditions associated with elevated peroxynitrite can be diagnosed. Also provided are compounds of formula (I) for use in the methods.",
author = "Tony James and Steven Bull and Xiaolong Sun",
year = "2019",
language = "English",
type = "Patent",
note = "WO 2015/128622 (EP3110827, US2017059573)",

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T1 - METHOD OF DETECTING PEROXYNITRITE USING A COMPLEX OF A SACCHARIDE AND AN ARYLBORONATE-BASED FLUORESCENT PROBE

AU - James, Tony

AU - Bull, Steven

AU - Sun, Xiaolong

PY - 2019

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N2 - A method of detecting peroxynitrite in a sample is described comprising the steps of: (a) providing a complex of a saccharide with an aryl boronate compound of formula (I): Fp - L1 - Z - L2 - Ar - B(OH)2 (I) wherein: Fp comprises a fluorophore; L1 and L2 are linker groups; Z is a fluorescence switch; and Ar is optionally substituted aryl; (b) contacting said aryl boronate- saccharide complex with said sample, whereby peroxynitrite in said sample cleaves said aryl boronate- saccharide complex to produce a compound of formula (II): F - L1 - Z - L2 - Ar - OH (II); and (c) detecting a decrease in a fluorescence intensity of said fluorophore resulting from said cleavage reaction in step (b). Peroxynitrite reacts quantitatively, rapidly, and selectively in step (b) of the reaction, whereby medical conditions associated with elevated peroxynitrite can be diagnosed. Also provided are compounds of formula (I) for use in the methods.

AB - A method of detecting peroxynitrite in a sample is described comprising the steps of: (a) providing a complex of a saccharide with an aryl boronate compound of formula (I): Fp - L1 - Z - L2 - Ar - B(OH)2 (I) wherein: Fp comprises a fluorophore; L1 and L2 are linker groups; Z is a fluorescence switch; and Ar is optionally substituted aryl; (b) contacting said aryl boronate- saccharide complex with said sample, whereby peroxynitrite in said sample cleaves said aryl boronate- saccharide complex to produce a compound of formula (II): F - L1 - Z - L2 - Ar - OH (II); and (c) detecting a decrease in a fluorescence intensity of said fluorophore resulting from said cleavage reaction in step (b). Peroxynitrite reacts quantitatively, rapidly, and selectively in step (b) of the reaction, whereby medical conditions associated with elevated peroxynitrite can be diagnosed. Also provided are compounds of formula (I) for use in the methods.

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