Cell extracts containing soluble methane monooxygenase from Methylococcus capsulatus, Methylosinus sporium and Methylosinus trichosporium converted 3-methylcyclohexene to predominantly cis-4-methylcyclohex-2-enol, contrary to an earlier suggestion of rearrangement products. In the case of M. capsulatus extracts, this comprised 90% of the total products. The product mixture was consistent with predictions based on chemical reactivity of the substrate, steric effects in the substrate and products and enzymic constraints operating in concert.
- Hydroxylation stereospecificity
- Methylococcus capsulatus
- Methylosinus sporium
- Methylosinus trichosporium
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