Metal–organic framework (MOF) hybridized gold nanoparticles as a bifunctional nanozyme for glucose sensing

Pei Hong Tong; Jing-Jing Wang; Xi Le Hu; Tony James; Xiao-Peng He

doi:10.1039/d3sc02598e

Metal–organic framework (MOF) hybridized gold nanoparticles as a bifunctional nanozyme for glucose sensing

Pei Hong Tong
, Jing-Jing Wang
, Xi Le Hu
, Tony James
, Xiao-Peng He

East China University of Science and Technology
The International Cooperation Laboratory on Signal Transduction

Research output: Contribution to journal › Article › peer-review

101 Citations (SciVal)

Abstract

Inspired by natural enzymes that possess multiple catalytic activities, here we develop a bifunctional metal–organic frame-work (MOF) for biosensing applications. Ultrasmall gold nano-particles (AuNPs) are grown in the internal cavities of an iron (Fe) porphyrin-based MOF to produce a hybridized nanozyme, AuNPs@PCN-224(Fe), in which AuNPs and PCN-224(Fe) exhibit the catalytic activity of glucose oxidase (GOx) and horseradish peroxidase (HRP), respectively. We established that the bifunctional nanozyme was capable of a cascade reaction to generate hydrogen peroxide in the presence of D-glucose and oxygen in situ, and subsequently activate a colorimetric or chemiluminescent substrate through HRP-mimicking catalytic activity. The nanozyme was selective over a range of other saccharides, and 93% of the catalytic activity was retained after being recycled five times.

Original language	English
Pages (from-to)	7762-7769
Number of pages	8
Journal	Chemical Science
Volume	14
Issue number	28
Early online date	22 Jun 2023
DOIs	https://doi.org/10.1039/d3sc02598e
Publication status	Published - 22 Jun 2023

Data Availability Statement

All experimental and data associated with this article have been included in the main text and ESI.

Funding

The authors thank the Natural National Science Foundation of China (No. 92253306), the Fundamental Research Funds for the Central Universities (222201717003), the Programme of Introducing Talents of Discipline to Universities (B16017), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering. TDJ wishes to thank the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). The Research Center of Analysis and Test of East China University of Science and Technology is gratefully acknowledged for assistance in analytical experiments.

Access to Document

10.1039/d3sc02598eLicence: CC BY

Cite this

@article{2f729003ea2e4a9688ad07f5cefc7e00,

title = "Metal{\textendash}organic framework (MOF) hybridized gold nanoparticles as a bifunctional nanozyme for glucose sensing",

abstract = "Inspired by natural enzymes that possess multiple catalytic activities, here we develop a bifunctional metal{\textendash}organic frame-work (MOF) for biosensing applications. Ultrasmall gold nano-particles (AuNPs) are grown in the internal cavities of an iron (Fe) porphyrin-based MOF to produce a hybridized nanozyme, AuNPs@PCN-224(Fe), in which AuNPs and PCN-224(Fe) exhibit the catalytic activity of glucose oxidase (GOx) and horseradish peroxidase (HRP), respectively. We established that the bifunctional nanozyme was capable of a cascade reaction to generate hydrogen peroxide in the presence of D-glucose and oxygen in situ, and subsequently activate a colorimetric or chemiluminescent substrate through HRP-mimicking catalytic activity. The nanozyme was selective over a range of other saccharides, and 93\% of the catalytic activity was retained after being recycled five times.",

author = "Tong, \{Pei Hong\} and Jing-Jing Wang and Hu, \{Xi Le\} and Tony James and Xiao-Peng He",

year = "2023",

month = jun,

day = "22",

doi = "10.1039/d3sc02598e",

language = "English",

volume = "14",

pages = "7762--7769",

journal = "Chemical Science",

issn = "2041-6520",

publisher = "Royal Society of Chemistry",

number = "28",

}

TY - JOUR

T1 - Metal–organic framework (MOF) hybridized gold nanoparticles as a bifunctional nanozyme for glucose sensing

AU - Tong, Pei Hong

AU - Wang, Jing-Jing

AU - Hu, Xi Le

AU - James, Tony

AU - He, Xiao-Peng

PY - 2023/6/22

Y1 - 2023/6/22

N2 - Inspired by natural enzymes that possess multiple catalytic activities, here we develop a bifunctional metal–organic frame-work (MOF) for biosensing applications. Ultrasmall gold nano-particles (AuNPs) are grown in the internal cavities of an iron (Fe) porphyrin-based MOF to produce a hybridized nanozyme, AuNPs@PCN-224(Fe), in which AuNPs and PCN-224(Fe) exhibit the catalytic activity of glucose oxidase (GOx) and horseradish peroxidase (HRP), respectively. We established that the bifunctional nanozyme was capable of a cascade reaction to generate hydrogen peroxide in the presence of D-glucose and oxygen in situ, and subsequently activate a colorimetric or chemiluminescent substrate through HRP-mimicking catalytic activity. The nanozyme was selective over a range of other saccharides, and 93% of the catalytic activity was retained after being recycled five times.

AB - Inspired by natural enzymes that possess multiple catalytic activities, here we develop a bifunctional metal–organic frame-work (MOF) for biosensing applications. Ultrasmall gold nano-particles (AuNPs) are grown in the internal cavities of an iron (Fe) porphyrin-based MOF to produce a hybridized nanozyme, AuNPs@PCN-224(Fe), in which AuNPs and PCN-224(Fe) exhibit the catalytic activity of glucose oxidase (GOx) and horseradish peroxidase (HRP), respectively. We established that the bifunctional nanozyme was capable of a cascade reaction to generate hydrogen peroxide in the presence of D-glucose and oxygen in situ, and subsequently activate a colorimetric or chemiluminescent substrate through HRP-mimicking catalytic activity. The nanozyme was selective over a range of other saccharides, and 93% of the catalytic activity was retained after being recycled five times.

UR - https://www.scopus.com/pages/publications/85165200114

U2 - 10.1039/d3sc02598e

DO - 10.1039/d3sc02598e

M3 - Article

SN - 2041-6520

VL - 14

SP - 7762

EP - 7769

JO - Chemical Science

JF - Chemical Science

IS - 28

ER -

Metal–organic framework (MOF) hybridized gold nanoparticles as a bifunctional nanozyme for glucose sensing

Abstract

Data Availability Statement

Funding

Access to Document

Other files and links

Fingerprint

Cite this