Abstract
Inspired by natural enzymes that possess multiple catalytic activities, here we develop a bifunctional metal–organic frame-work (MOF) for biosensing applications. Ultrasmall gold nano-particles (AuNPs) are grown in the internal cavities of an iron (Fe) porphyrin-based MOF to produce a hybridized nanozyme, AuNPs@PCN-224(Fe), in which AuNPs and PCN-224(Fe) exhibit the catalytic activity of glucose oxidase (GOx) and horseradish peroxidase (HRP), respectively. We established that the bifunctional nanozyme was capable of a cascade reaction to generate hydrogen peroxide in the presence of D-glucose and oxygen in situ, and subsequently activate a colorimetric or chemiluminescent substrate through HRP-mimicking catalytic activity. The nanozyme was selective over a range of other saccharides, and 93% of the catalytic activity was retained after being recycled five times.
| Original language | English |
|---|---|
| Pages (from-to) | 7762-7769 |
| Number of pages | 8 |
| Journal | Chemical Science |
| Volume | 14 |
| Issue number | 28 |
| Early online date | 22 Jun 2023 |
| DOIs | |
| Publication status | Published - 22 Jun 2023 |
Data Availability Statement
All experimental and data associated with this article have been included in the main text and ESI.Funding
The authors thank the Natural National Science Foundation of China (No. 92253306), the Fundamental Research Funds for the Central Universities (222201717003), the Programme of Introducing Talents of Discipline to Universities (B16017), and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering. TDJ wishes to thank the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). The Research Center of Analysis and Test of East China University of Science and Technology is gratefully acknowledged for assistance in analytical experiments.