Mechanical tomography of human corneocytes with a nanoneedle

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Abstract

Atomic force microscopy (AFM) can image biological samples and characterize their mechanical properties. However, the low aspect ratio of standard AFM probes typically limits these measurements to surface properties. Here, the intracellular mechanical behavior of human corneocytes is determined using ‘‘nanoneedle’’ AFM probes. The method evaluates the forces experienced by a nanoneedle as it is pushed into and then retracted from the cell. Indentation loops yield the stiffness profile and information on the elastic and nonelastic mechanical properties at a specific depth below the surface of the corneocytes. A clear difference between the softer B50-nm-thick external layer and the more rigid internal structure of corneocytes is apparent, which is consistent with the current understanding of the structure of these cells. There are also significant variations in the mechanical properties of corneocytes from different volunteers. The small diameter of the nanoneedle allows this ‘‘mechanical tomography’’ to be performed with high spatial resolution, potentially offering an opportunity to detect biomechanical changes in corneocytes because of, e.g., environmental factors, aging, or dermatological pathologies.
Original languageEnglish
Pages (from-to)1565-1571
Number of pages7
JournalJournal Of Investigative Dermatology
Volume133
Issue number6
Early online date6 Dec 2012
DOIs
Publication statusPublished - Jun 2013

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Nanoneedles
Atomic Force Microscopy
Tomography
Atomic force microscopy
Mechanical properties
Surface Properties
Pathology
Indentation
Surface properties
Aspect ratio
Volunteers
Aging of materials
Stiffness

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Mechanical tomography of human corneocytes with a nanoneedle. / Beard, James D; Guy, Richard H; Gordeev, Sergey N.

In: Journal Of Investigative Dermatology, Vol. 133, No. 6, 06.2013, p. 1565-1571.

Research output: Contribution to journalArticle

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