Mannose-resistant Proteus-like and P. mirabilis fimbriae have specific and additive roles in P. mirabilis urinary tract infections

Pablo Zunino, Vanessa Sosa, Geraldine Schlapp, Andrew G Allen, Andrew Preston, Duncan J Maskell

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Proteus mirabilis is an important uropathogen that can cause complicated urinary tract infections (UTI). It produces several types of fimbriae, including mannose-resistant Proteus-like (MR/P) fimbriae and P. mirabilis fimbriae (PMF). Previously, we determined that these fimbriae affect the ability of P. mirabilis to colonize the urinary tract. The objective of this study was to analyse the effect of the simultaneous lack of P. mirabilis MR/P and PMF fimbriae in UTI pathogenesis. A double mutant lacking both fimbriae was generated by allelic replacement mutagenesis. This mutant was characterized genetically and phenotypically, and tested using an in vitro uroepithelial cell adhesion assay and the ascending UTI murine model. In vitro adhesion to uroepithelial cells by the P. mirabilis pmfA/mrpA-D mutant was reduced when compared with the wild-type, although no significant differences were observed when it was compared with the single mrpA-D and pmfA mutants. However, in vivo assays showed that colonization of kidneys and bladders by the P. mirabilis pmfA/mrpA-D mutant was significantly reduced when compared with the wild-type and both single mutants. These results indicate that, although redundancy can occur, MR/P and PMF fimbriae have specific and additive roles in P. mirabilis UTI.
Original languageEnglish
Pages (from-to)125-133
Number of pages9
JournalFEMS Immunology and Medical Microbiology
Volume51
Issue number1
DOIs
Publication statusPublished - 2007

Fingerprint

Mirabilis
Proteus mirabilis
Proteus
Mannose
Urinary Tract Infections
Urinary Tract
Cell Adhesion
Mutagenesis
Urinary Bladder

Cite this

Mannose-resistant Proteus-like and P. mirabilis fimbriae have specific and additive roles in P. mirabilis urinary tract infections. / Zunino, Pablo; Sosa, Vanessa; Schlapp, Geraldine; Allen, Andrew G; Preston, Andrew; Maskell, Duncan J.

In: FEMS Immunology and Medical Microbiology, Vol. 51, No. 1, 2007, p. 125-133.

Research output: Contribution to journalArticle

Zunino, Pablo ; Sosa, Vanessa ; Schlapp, Geraldine ; Allen, Andrew G ; Preston, Andrew ; Maskell, Duncan J. / Mannose-resistant Proteus-like and P. mirabilis fimbriae have specific and additive roles in P. mirabilis urinary tract infections. In: FEMS Immunology and Medical Microbiology. 2007 ; Vol. 51, No. 1. pp. 125-133.
@article{01130797041140f1b9c1786c8452b7cc,
title = "Mannose-resistant Proteus-like and P. mirabilis fimbriae have specific and additive roles in P. mirabilis urinary tract infections",
abstract = "Proteus mirabilis is an important uropathogen that can cause complicated urinary tract infections (UTI). It produces several types of fimbriae, including mannose-resistant Proteus-like (MR/P) fimbriae and P. mirabilis fimbriae (PMF). Previously, we determined that these fimbriae affect the ability of P. mirabilis to colonize the urinary tract. The objective of this study was to analyse the effect of the simultaneous lack of P. mirabilis MR/P and PMF fimbriae in UTI pathogenesis. A double mutant lacking both fimbriae was generated by allelic replacement mutagenesis. This mutant was characterized genetically and phenotypically, and tested using an in vitro uroepithelial cell adhesion assay and the ascending UTI murine model. In vitro adhesion to uroepithelial cells by the P. mirabilis pmfA/mrpA-D mutant was reduced when compared with the wild-type, although no significant differences were observed when it was compared with the single mrpA-D and pmfA mutants. However, in vivo assays showed that colonization of kidneys and bladders by the P. mirabilis pmfA/mrpA-D mutant was significantly reduced when compared with the wild-type and both single mutants. These results indicate that, although redundancy can occur, MR/P and PMF fimbriae have specific and additive roles in P. mirabilis UTI.",
author = "Pablo Zunino and Vanessa Sosa and Geraldine Schlapp and Allen, {Andrew G} and Andrew Preston and Maskell, {Duncan J}",
year = "2007",
doi = "10.1111/j.1574-695X.2007.00285.x",
language = "English",
volume = "51",
pages = "125--133",
journal = "FEMS Immunology and Medical Microbiology",
issn = "0928-8244",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Mannose-resistant Proteus-like and P. mirabilis fimbriae have specific and additive roles in P. mirabilis urinary tract infections

AU - Zunino, Pablo

AU - Sosa, Vanessa

AU - Schlapp, Geraldine

AU - Allen, Andrew G

AU - Preston, Andrew

AU - Maskell, Duncan J

PY - 2007

Y1 - 2007

N2 - Proteus mirabilis is an important uropathogen that can cause complicated urinary tract infections (UTI). It produces several types of fimbriae, including mannose-resistant Proteus-like (MR/P) fimbriae and P. mirabilis fimbriae (PMF). Previously, we determined that these fimbriae affect the ability of P. mirabilis to colonize the urinary tract. The objective of this study was to analyse the effect of the simultaneous lack of P. mirabilis MR/P and PMF fimbriae in UTI pathogenesis. A double mutant lacking both fimbriae was generated by allelic replacement mutagenesis. This mutant was characterized genetically and phenotypically, and tested using an in vitro uroepithelial cell adhesion assay and the ascending UTI murine model. In vitro adhesion to uroepithelial cells by the P. mirabilis pmfA/mrpA-D mutant was reduced when compared with the wild-type, although no significant differences were observed when it was compared with the single mrpA-D and pmfA mutants. However, in vivo assays showed that colonization of kidneys and bladders by the P. mirabilis pmfA/mrpA-D mutant was significantly reduced when compared with the wild-type and both single mutants. These results indicate that, although redundancy can occur, MR/P and PMF fimbriae have specific and additive roles in P. mirabilis UTI.

AB - Proteus mirabilis is an important uropathogen that can cause complicated urinary tract infections (UTI). It produces several types of fimbriae, including mannose-resistant Proteus-like (MR/P) fimbriae and P. mirabilis fimbriae (PMF). Previously, we determined that these fimbriae affect the ability of P. mirabilis to colonize the urinary tract. The objective of this study was to analyse the effect of the simultaneous lack of P. mirabilis MR/P and PMF fimbriae in UTI pathogenesis. A double mutant lacking both fimbriae was generated by allelic replacement mutagenesis. This mutant was characterized genetically and phenotypically, and tested using an in vitro uroepithelial cell adhesion assay and the ascending UTI murine model. In vitro adhesion to uroepithelial cells by the P. mirabilis pmfA/mrpA-D mutant was reduced when compared with the wild-type, although no significant differences were observed when it was compared with the single mrpA-D and pmfA mutants. However, in vivo assays showed that colonization of kidneys and bladders by the P. mirabilis pmfA/mrpA-D mutant was significantly reduced when compared with the wild-type and both single mutants. These results indicate that, although redundancy can occur, MR/P and PMF fimbriae have specific and additive roles in P. mirabilis UTI.

UR - http://dx.doi.org/10.1111/j.1574-695X.2007.00285.x

U2 - 10.1111/j.1574-695X.2007.00285.x

DO - 10.1111/j.1574-695X.2007.00285.x

M3 - Article

VL - 51

SP - 125

EP - 133

JO - FEMS Immunology and Medical Microbiology

JF - FEMS Immunology and Medical Microbiology

SN - 0928-8244

IS - 1

ER -