Mammary cancer in transgenic mice expressing insulin-like growth factor II (IGF-II)

P Bates, Rebecca Fisher, A Ward, L Richardson, D J Hill, C F Graham

Research output: Contribution to journalArticle

Abstract

The effect of insulin-like growth factor II (IGF-II) on tumour development in the mouse mammary gland was studied. To promote extra IGF-II expression in the mammary gland, sheep beta-lactoglobulin regulatory elements were attached to the coding regions of the mouse Igf-2 gene and injected into the pronuclei of mouse zygotes. Mammary tumours developed in each of the four independent lines of mice which expressed transgene IGF-II in the gland. Tumours from two of the lines grew after transplantation to both male and female hosts. Primary tumours contained stromal and epithelial regions, but the tumours were dominated by mammary adenocarcinoma after transplantation. The tumours expressed high levels of Igf-2 mRNA transcribed from the integrated transgenes.

Original languageEnglish
Pages (from-to)1189-93
Number of pages5
JournalBritish Journal of Cancer
Volume72
Issue number5
Publication statusPublished - Nov 1995

Fingerprint

Insulin-Like Growth Factor II
Transgenic Mice
Breast Neoplasms
Human Mammary Glands
Neoplasms
Transgenes
Transplantation
Lactoglobulins
Zygote
Sheep
Adenocarcinoma
Breast
Messenger RNA
Genes

Keywords

  • Adenocarcinoma
  • Animals
  • Cell Transformation, Neoplastic
  • Crosses, Genetic
  • Female
  • Gene Expression Regulation
  • Insulin-Like Growth Factor II
  • Lactoglobulins
  • Male
  • Mammary Glands, Animal
  • Mammary Neoplasms, Experimental
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Transgenic
  • Neoplasm Proteins
  • Regulatory Sequences, Nucleic Acid
  • Sheep
  • Transgenes
  • Comparative Study
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Bates, P., Fisher, R., Ward, A., Richardson, L., Hill, D. J., & Graham, C. F. (1995). Mammary cancer in transgenic mice expressing insulin-like growth factor II (IGF-II). British Journal of Cancer, 72(5), 1189-93.

Mammary cancer in transgenic mice expressing insulin-like growth factor II (IGF-II). / Bates, P; Fisher, Rebecca; Ward, A; Richardson, L; Hill, D J; Graham, C F.

In: British Journal of Cancer, Vol. 72, No. 5, 11.1995, p. 1189-93.

Research output: Contribution to journalArticle

Bates, P, Fisher, R, Ward, A, Richardson, L, Hill, DJ & Graham, CF 1995, 'Mammary cancer in transgenic mice expressing insulin-like growth factor II (IGF-II)', British Journal of Cancer, vol. 72, no. 5, pp. 1189-93.
Bates P, Fisher R, Ward A, Richardson L, Hill DJ, Graham CF. Mammary cancer in transgenic mice expressing insulin-like growth factor II (IGF-II). British Journal of Cancer. 1995 Nov;72(5):1189-93.
Bates, P ; Fisher, Rebecca ; Ward, A ; Richardson, L ; Hill, D J ; Graham, C F. / Mammary cancer in transgenic mice expressing insulin-like growth factor II (IGF-II). In: British Journal of Cancer. 1995 ; Vol. 72, No. 5. pp. 1189-93.
@article{c02956bb01a7479496dc889468af1666,
title = "Mammary cancer in transgenic mice expressing insulin-like growth factor II (IGF-II)",
abstract = "The effect of insulin-like growth factor II (IGF-II) on tumour development in the mouse mammary gland was studied. To promote extra IGF-II expression in the mammary gland, sheep beta-lactoglobulin regulatory elements were attached to the coding regions of the mouse Igf-2 gene and injected into the pronuclei of mouse zygotes. Mammary tumours developed in each of the four independent lines of mice which expressed transgene IGF-II in the gland. Tumours from two of the lines grew after transplantation to both male and female hosts. Primary tumours contained stromal and epithelial regions, but the tumours were dominated by mammary adenocarcinoma after transplantation. The tumours expressed high levels of Igf-2 mRNA transcribed from the integrated transgenes.",
keywords = "Adenocarcinoma, Animals, Cell Transformation, Neoplastic, Crosses, Genetic, Female, Gene Expression Regulation, Insulin-Like Growth Factor II, Lactoglobulins, Male, Mammary Glands, Animal, Mammary Neoplasms, Experimental, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Transgenic, Neoplasm Proteins, Regulatory Sequences, Nucleic Acid, Sheep, Transgenes, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't",
author = "P Bates and Rebecca Fisher and A Ward and L Richardson and Hill, {D J} and Graham, {C F}",
year = "1995",
month = "11",
language = "English",
volume = "72",
pages = "1189--93",
journal = "British Journal of Cancer",
issn = "0007-0920",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Mammary cancer in transgenic mice expressing insulin-like growth factor II (IGF-II)

AU - Bates, P

AU - Fisher, Rebecca

AU - Ward, A

AU - Richardson, L

AU - Hill, D J

AU - Graham, C F

PY - 1995/11

Y1 - 1995/11

N2 - The effect of insulin-like growth factor II (IGF-II) on tumour development in the mouse mammary gland was studied. To promote extra IGF-II expression in the mammary gland, sheep beta-lactoglobulin regulatory elements were attached to the coding regions of the mouse Igf-2 gene and injected into the pronuclei of mouse zygotes. Mammary tumours developed in each of the four independent lines of mice which expressed transgene IGF-II in the gland. Tumours from two of the lines grew after transplantation to both male and female hosts. Primary tumours contained stromal and epithelial regions, but the tumours were dominated by mammary adenocarcinoma after transplantation. The tumours expressed high levels of Igf-2 mRNA transcribed from the integrated transgenes.

AB - The effect of insulin-like growth factor II (IGF-II) on tumour development in the mouse mammary gland was studied. To promote extra IGF-II expression in the mammary gland, sheep beta-lactoglobulin regulatory elements were attached to the coding regions of the mouse Igf-2 gene and injected into the pronuclei of mouse zygotes. Mammary tumours developed in each of the four independent lines of mice which expressed transgene IGF-II in the gland. Tumours from two of the lines grew after transplantation to both male and female hosts. Primary tumours contained stromal and epithelial regions, but the tumours were dominated by mammary adenocarcinoma after transplantation. The tumours expressed high levels of Igf-2 mRNA transcribed from the integrated transgenes.

KW - Adenocarcinoma

KW - Animals

KW - Cell Transformation, Neoplastic

KW - Crosses, Genetic

KW - Female

KW - Gene Expression Regulation

KW - Insulin-Like Growth Factor II

KW - Lactoglobulins

KW - Male

KW - Mammary Glands, Animal

KW - Mammary Neoplasms, Experimental

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Inbred CBA

KW - Mice, Transgenic

KW - Neoplasm Proteins

KW - Regulatory Sequences, Nucleic Acid

KW - Sheep

KW - Transgenes

KW - Comparative Study

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

VL - 72

SP - 1189

EP - 1193

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

IS - 5

ER -