Mammalian phospholipase C zeta induces oocyte activation from the sperm perinuclear matrix

S Fujimoto, N Yoshida, T Fukui, M Amanai, T Isobe, C Itagaki, T Izumi, Anthony C F Perry

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Mammalian sperm-borne oocyte activating factor (SOAF) induces oocyte activation from a compartment that engages the oocyte cytoplasm, but it is not known how. A SOAF-containing extract (SE) was solubilized from the submembrane perinuclear matrix, a domain that enters the egg. SE initiated activation sufficient for full development. Microinjection coupled to tandem mass spectrometry enabled functional correlation profiling of fractionated SE without a priori assumptions about its chemical nature. Phospholipase C-zeta (PLCzeta) correlated absolutely with activating ability. Immunoblotting confirmed this and showed that the perinuclear matrix is the major site of 72-kDa PLCzeta. Oocyte activation was efficiently induced by 1.25 fg of sperm PLCzeta, corresponding to a fraction of one sperm equivalent (similar to0.03). Immunofluorescence microscopy localized sperm head PLCzeta to a post-acrosomal region that becomes rapidly exposed to the ooplasm following gamete fusion. This multifaceted approach suggests a mechanism by which PLCzeta originates from an oocyte-penetrating assembly the sperm perinuclear matrix-to induce mammalian oocyte activation at fertilization.
Original languageEnglish
Pages (from-to)370-383
Number of pages14
JournalDevelopmental Biology
Volume274
Issue number2
DOIs
Publication statusPublished - 2004

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Type C Phospholipases
Oocytes
Spermatozoa
Sperm Head
Microinjections
Tandem Mass Spectrometry
Fluorescence Microscopy
Immunoblotting
Fertilization
Germ Cells
Ovum
Cytoplasm

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Mammalian phospholipase C zeta induces oocyte activation from the sperm perinuclear matrix. / Fujimoto, S; Yoshida, N; Fukui, T; Amanai, M; Isobe, T; Itagaki, C; Izumi, T; Perry, Anthony C F.

In: Developmental Biology, Vol. 274, No. 2, 2004, p. 370-383.

Research output: Contribution to journalArticle

Fujimoto, S, Yoshida, N, Fukui, T, Amanai, M, Isobe, T, Itagaki, C, Izumi, T & Perry, ACF 2004, 'Mammalian phospholipase C zeta induces oocyte activation from the sperm perinuclear matrix', Developmental Biology, vol. 274, no. 2, pp. 370-383. https://doi.org/10.1016/j.ydbio.2004.07.025
Fujimoto, S ; Yoshida, N ; Fukui, T ; Amanai, M ; Isobe, T ; Itagaki, C ; Izumi, T ; Perry, Anthony C F. / Mammalian phospholipase C zeta induces oocyte activation from the sperm perinuclear matrix. In: Developmental Biology. 2004 ; Vol. 274, No. 2. pp. 370-383.
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AU - Itagaki, C

AU - Izumi, T

AU - Perry, Anthony C F

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