Mammalian phospholipase C zeta induces oocyte activation from the sperm perinuclear matrix

S Fujimoto, N Yoshida, T Fukui, M Amanai, T Isobe, C Itagaki, T Izumi, Anthony C F Perry

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Mammalian sperm-borne oocyte activating factor (SOAF) induces oocyte activation from a compartment that engages the oocyte cytoplasm, but it is not known how. A SOAF-containing extract (SE) was solubilized from the submembrane perinuclear matrix, a domain that enters the egg. SE initiated activation sufficient for full development. Microinjection coupled to tandem mass spectrometry enabled functional correlation profiling of fractionated SE without a priori assumptions about its chemical nature. Phospholipase C-zeta (PLCzeta) correlated absolutely with activating ability. Immunoblotting confirmed this and showed that the perinuclear matrix is the major site of 72-kDa PLCzeta. Oocyte activation was efficiently induced by 1.25 fg of sperm PLCzeta, corresponding to a fraction of one sperm equivalent (similar to0.03). Immunofluorescence microscopy localized sperm head PLCzeta to a post-acrosomal region that becomes rapidly exposed to the ooplasm following gamete fusion. This multifaceted approach suggests a mechanism by which PLCzeta originates from an oocyte-penetrating assembly the sperm perinuclear matrix-to induce mammalian oocyte activation at fertilization.
Original languageEnglish
Pages (from-to)370-383
Number of pages14
JournalDevelopmental Biology
Volume274
Issue number2
DOIs
Publication statusPublished - 2004

Fingerprint Dive into the research topics of 'Mammalian phospholipase C zeta induces oocyte activation from the sperm perinuclear matrix'. Together they form a unique fingerprint.

  • Cite this