Lysosomal tracking with a cationic naphthalimide using multiphoton fluorescence lifetime imaging microscopy

Meng Li, Haobo Ge, Vincenzo Mirabello, Rory L. Arrowsmith, Gabriele Kociok-Kohn, Stanley W Botchway, Weihong Zhu, Sofia I. Pascu, Tony D. James

Research output: Contribution to journalArticle

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Abstract

A naphthalimide-based chemosensing motif turns ON the fluorescence emission in solution in the presence of aqueous iron(III) chloride, and maintains this property in living cancer cells. The emission response to Fe(III) ions occurs simultaneously with a change in pH. The protonation of methyl piperazine-conjugated naphthalimide promotes its lysosomal localisation as assessed by co-localisation tests and fluorescence lifetime imaging microscopy (FLIM) in vitro.
Original languageEnglish
Pages (from-to)11161-11164
JournalChemical Communications
Volume53
Issue number81
Early online date1 Sep 2017
DOIs
Publication statusPublished - 18 Oct 2017

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Naphthalimides
Microscopic examination
Fluorescence
Imaging techniques
Protonation
Chlorides
Iron
Cells
Ions

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Lysosomal tracking with a cationic naphthalimide using multiphoton fluorescence lifetime imaging microscopy. / Li, Meng; Ge, Haobo; Mirabello, Vincenzo; Arrowsmith, Rory L.; Kociok-Kohn, Gabriele; Botchway, Stanley W; Zhu, Weihong; Pascu, Sofia I.; James, Tony D.

In: Chemical Communications, Vol. 53, No. 81, 18.10.2017, p. 11161-11164.

Research output: Contribution to journalArticle

Li, Meng ; Ge, Haobo ; Mirabello, Vincenzo ; Arrowsmith, Rory L. ; Kociok-Kohn, Gabriele ; Botchway, Stanley W ; Zhu, Weihong ; Pascu, Sofia I. ; James, Tony D. / Lysosomal tracking with a cationic naphthalimide using multiphoton fluorescence lifetime imaging microscopy. In: Chemical Communications. 2017 ; Vol. 53, No. 81. pp. 11161-11164.
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