Low-cost lipid production by an oleaginous yeast cultured in non-sterile conditions using model waste resources

F Santomauro, Fraeya Whiffin, R J Scott, C Chuck

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Background
The yeast Metschnikowia pulcherrima, previously utilised as a biological control agent, was evaluated for its potential to produce lipids for biofuel production.
Results
Cultivation in low cost non-sterile conditions was achieved by exploiting its ability to grow at low temperature and pH and to produce natural antimicrobial compounds. Although not previously classified as oleaginous, a combination of low temperature and restricted nutrient availability triggered high levels of oil production in M. pulcherrima cultures. This regime was designed to trigger the sporulation process but prevent its completion to allow the accumulation of a subset of a normally transitional, but oil-rich, ‘pulcherrima’ cell type. This approach resulted in yields of up to 40% lipid, which compares favourably with other oleaginous microbes. We also demonstrate that M. pulcherrima metabolises glycerol and a diverse range of other sugars, suggesting that heterogeneous biomass could provide a suitable carbon source. M. pulcherrima also grows well in a minimal media containing no yeast extract. Finally, we demonstrate the potential of the yeast to produce lipids inexpensively on an industrial scale by culturing the yeast in a 500L, open air, tank reactor without any significant contamination.
Conclusions
The production of antimicrobial compounds coupled to efficient growth at low temperature and pH enables culture of this oleaginous yeast in inexpensive, non-sterile conditions providing a potential route to economic biofuel production.
LanguageEnglish
Article number34
Pages34-43
Number of pages10
JournalBiotechnology for Biofuels
Volume7
DOIs
StatusPublished - 4 Mar 2014

Fingerprint

Yeast
Lipids
yeast
Yeasts
lipid
Costs and Cost Analysis
resource
cost
Biofuels
Costs
biofuel
Temperature
Metschnikowia
Oils
Biological Control Agents
sporulation
nutrient availability
Glycerol
Sugars
biological control

Keywords

  • biodiesel
  • lipid
  • yeast
  • heterotrophic
  • pilot scale
  • waste

Cite this

Low-cost lipid production by an oleaginous yeast cultured in non-sterile conditions using model waste resources. / Santomauro, F; Whiffin, Fraeya; Scott, R J; Chuck, C.

In: Biotechnology for Biofuels, Vol. 7, 34, 04.03.2014, p. 34-43.

Research output: Contribution to journalArticle

@article{2471a4c8228c45bfbb1e04abd897af2e,
title = "Low-cost lipid production by an oleaginous yeast cultured in non-sterile conditions using model waste resources",
abstract = "Background The yeast Metschnikowia pulcherrima, previously utilised as a biological control agent, was evaluated for its potential to produce lipids for biofuel production. ResultsCultivation in low cost non-sterile conditions was achieved by exploiting its ability to grow at low temperature and pH and to produce natural antimicrobial compounds. Although not previously classified as oleaginous, a combination of low temperature and restricted nutrient availability triggered high levels of oil production in M. pulcherrima cultures. This regime was designed to trigger the sporulation process but prevent its completion to allow the accumulation of a subset of a normally transitional, but oil-rich, ‘pulcherrima’ cell type. This approach resulted in yields of up to 40{\%} lipid, which compares favourably with other oleaginous microbes. We also demonstrate that M. pulcherrima metabolises glycerol and a diverse range of other sugars, suggesting that heterogeneous biomass could provide a suitable carbon source. M. pulcherrima also grows well in a minimal media containing no yeast extract. Finally, we demonstrate the potential of the yeast to produce lipids inexpensively on an industrial scale by culturing the yeast in a 500L, open air, tank reactor without any significant contamination. ConclusionsThe production of antimicrobial compounds coupled to efficient growth at low temperature and pH enables culture of this oleaginous yeast in inexpensive, non-sterile conditions providing a potential route to economic biofuel production.",
keywords = "biodiesel, lipid, yeast, heterotrophic, pilot scale, waste",
author = "F Santomauro and Fraeya Whiffin and Scott, {R J} and C Chuck",
year = "2014",
month = "3",
day = "4",
doi = "10.1186/1754-6834-7-34",
language = "English",
volume = "7",
pages = "34--43",
journal = "Biotechnology for Biofuels",
issn = "1754-6834",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Low-cost lipid production by an oleaginous yeast cultured in non-sterile conditions using model waste resources

AU - Santomauro, F

AU - Whiffin, Fraeya

AU - Scott, R J

AU - Chuck, C

PY - 2014/3/4

Y1 - 2014/3/4

N2 - Background The yeast Metschnikowia pulcherrima, previously utilised as a biological control agent, was evaluated for its potential to produce lipids for biofuel production. ResultsCultivation in low cost non-sterile conditions was achieved by exploiting its ability to grow at low temperature and pH and to produce natural antimicrobial compounds. Although not previously classified as oleaginous, a combination of low temperature and restricted nutrient availability triggered high levels of oil production in M. pulcherrima cultures. This regime was designed to trigger the sporulation process but prevent its completion to allow the accumulation of a subset of a normally transitional, but oil-rich, ‘pulcherrima’ cell type. This approach resulted in yields of up to 40% lipid, which compares favourably with other oleaginous microbes. We also demonstrate that M. pulcherrima metabolises glycerol and a diverse range of other sugars, suggesting that heterogeneous biomass could provide a suitable carbon source. M. pulcherrima also grows well in a minimal media containing no yeast extract. Finally, we demonstrate the potential of the yeast to produce lipids inexpensively on an industrial scale by culturing the yeast in a 500L, open air, tank reactor without any significant contamination. ConclusionsThe production of antimicrobial compounds coupled to efficient growth at low temperature and pH enables culture of this oleaginous yeast in inexpensive, non-sterile conditions providing a potential route to economic biofuel production.

AB - Background The yeast Metschnikowia pulcherrima, previously utilised as a biological control agent, was evaluated for its potential to produce lipids for biofuel production. ResultsCultivation in low cost non-sterile conditions was achieved by exploiting its ability to grow at low temperature and pH and to produce natural antimicrobial compounds. Although not previously classified as oleaginous, a combination of low temperature and restricted nutrient availability triggered high levels of oil production in M. pulcherrima cultures. This regime was designed to trigger the sporulation process but prevent its completion to allow the accumulation of a subset of a normally transitional, but oil-rich, ‘pulcherrima’ cell type. This approach resulted in yields of up to 40% lipid, which compares favourably with other oleaginous microbes. We also demonstrate that M. pulcherrima metabolises glycerol and a diverse range of other sugars, suggesting that heterogeneous biomass could provide a suitable carbon source. M. pulcherrima also grows well in a minimal media containing no yeast extract. Finally, we demonstrate the potential of the yeast to produce lipids inexpensively on an industrial scale by culturing the yeast in a 500L, open air, tank reactor without any significant contamination. ConclusionsThe production of antimicrobial compounds coupled to efficient growth at low temperature and pH enables culture of this oleaginous yeast in inexpensive, non-sterile conditions providing a potential route to economic biofuel production.

KW - biodiesel

KW - lipid

KW - yeast

KW - heterotrophic

KW - pilot scale

KW - waste

U2 - 10.1186/1754-6834-7-34

DO - 10.1186/1754-6834-7-34

M3 - Article

VL - 7

SP - 34

EP - 43

JO - Biotechnology for Biofuels

T2 - Biotechnology for Biofuels

JF - Biotechnology for Biofuels

SN - 1754-6834

M1 - 34

ER -