Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells

Lauren Gwynne, Adam C. Sedgwick, Jordan E. Gardiner, George T. Williams, Gyoungmi Kim, Jean Yves Maillard, Toby Jenkins, Steven D. Bull, Jonathan L. Sessler, Juyoung Yoon, Tony D. James

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A long wavelength TCF-based fluorescent probe ( TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence "turn on" (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.

Original languageEnglish
Article number255
Pages (from-to)1-6
Number of pages6
JournalFrontiers in Chemistry
Volume7
Issue numberAPR
DOIs
Publication statusPublished - 30 Apr 2019

Keywords

  • Alkaline Phosphatase
  • Cell imaging
  • Colorimetric
  • Fluorescence
  • Reaction-based fluorescent probe

ASJC Scopus subject areas

  • Chemistry(all)

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