Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells

Lauren Gwynne; Adam C. Sedgwick; Jordan E. Gardiner; George T. Williams; Gyoungmi Kim; Jean Yves Maillard; Toby Jenkins; Steven D. Bull; Jonathan L. Sessler; Juyoung Yoon; Tony D. James

doi:10.3389/fchem.2019.00255

Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells

Lauren Gwynne, Adam C. Sedgwick, Jordan E. Gardiner, George T. Williams, Gyoungmi Kim, Jean Yves Maillard, Toby Jenkins, Steven D. Bull, Jonathan L. Sessler, Juyoung Yoon, Tony D. James

Research output: Contribution to journal › Article › peer-review

28 Citations (SciVal)

Abstract

A long wavelength TCF-based fluorescent probe ( TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence "turn on" (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.

Original language	English
Article number	255
Pages (from-to)	1-6
Number of pages	6
Journal	Frontiers in Chemistry
Volume	7
Issue number	APR
DOIs	https://doi.org/10.3389/fchem.2019.00255
Publication status	Published - 30 Apr 2019

Keywords

Alkaline Phosphatase
Cell imaging
Colorimetric
Fluorescence
Reaction-based fluorescent probe

ASJC Scopus subject areas

General Chemistry

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10.3389/fchem.2019.00255Licence: CC BY

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@article{83de4579e9784547851fbc51731292e5,

title = "Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells",

abstract = "A long wavelength TCF-based fluorescent probe ( TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence {"}turn on{"} (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems. ",

keywords = "Alkaline Phosphatase, Cell imaging, Colorimetric, Fluorescence, Reaction-based fluorescent probe",

author = "Lauren Gwynne and Sedgwick, {Adam C.} and Gardiner, {Jordan E.} and Williams, {George T.} and Gyoungmi Kim and Maillard, {Jean Yves} and Toby Jenkins and Bull, {Steven D.} and Sessler, {Jonathan L.} and Juyoung Yoon and James, {Tony D.}",

year = "2019",

month = apr,

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doi = "10.3389/fchem.2019.00255",

language = "English",

volume = "7",

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journal = "Frontiers in Chemistry",

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T1 - Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells

AU - Gwynne, Lauren

AU - Sedgwick, Adam C.

AU - Gardiner, Jordan E.

AU - Williams, George T.

AU - Kim, Gyoungmi

AU - Maillard, Jean Yves

AU - Jenkins, Toby

AU - Bull, Steven D.

AU - Sessler, Jonathan L.

AU - Yoon, Juyoung

AU - James, Tony D.

PY - 2019/4/30

Y1 - 2019/4/30

N2 - A long wavelength TCF-based fluorescent probe ( TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence "turn on" (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.

AB - A long wavelength TCF-based fluorescent probe ( TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence "turn on" (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.

KW - Alkaline Phosphatase

KW - Cell imaging

KW - Colorimetric

KW - Fluorescence

KW - Reaction-based fluorescent probe

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JO - Frontiers in Chemistry

JF - Frontiers in Chemistry

IS - APR

M1 - 255

ER -

Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells

Abstract

Keywords

ASJC Scopus subject areas

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Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

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