Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols

Adam C. Sedgwick, Jordan E. Gardiner, Gyoungmi Kim, Maksims Yevglevskis, Matthew D. Lloyd, A. Toby A. Jenkins, Steven D. Bull, Juyoung Yoon, Tony D. James

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Two 'turn on' TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found for TCFCl-GSH and a clear 'turn on' with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.

LanguageEnglish
Pages4786-4789
Number of pages4
JournalChemical Communications
Volume54
Issue number38
Early online date17 Apr 2018
DOIs
StatusPublished - 2018

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Sulfhydryl Compounds
Toxicity
Fluorescence
Drug therapy
Imaging techniques
Wavelength
Glutathione

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols. / Sedgwick, Adam C.; Gardiner, Jordan E.; Kim, Gyoungmi; Yevglevskis, Maksims; Lloyd, Matthew D.; Jenkins, A. Toby A.; Bull, Steven D.; Yoon, Juyoung; James, Tony D.

In: Chemical Communications, Vol. 54, No. 38, 2018, p. 4786-4789.

Research output: Contribution to journalArticle

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AU - Gardiner, Jordan E.

AU - Kim, Gyoungmi

AU - Yevglevskis, Maksims

AU - Lloyd, Matthew D.

AU - Jenkins, A. Toby A.

AU - Bull, Steven D.

AU - Yoon, Juyoung

AU - James, Tony D.

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