Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols

Adam C. Sedgwick; Jordan E. Gardiner; Gyoungmi Kim; Maksims Yevglevskis; Matthew D. Lloyd; A. Toby A. Jenkins; Steven D. Bull; Juyoung Yoon; Tony D. James

doi:10.1039/c8cc01661e

Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols

Adam C. Sedgwick, Jordan E. Gardiner, Gyoungmi Kim, Maksims Yevglevskis, Matthew D. Lloyd, A. Toby A. Jenkins, Steven D. Bull, Juyoung Yoon, Tony D. James

Ewha Womans University

Research output: Contribution to journal › Article › peer-review

66 Citations (SciVal)

Abstract

Two 'turn on' TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found for TCFCl-GSH and a clear 'turn on' with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.

Original language	English
Pages (from-to)	4786-4789
Number of pages	4
Journal	Chemical Communications
Volume	54
Issue number	38
Early online date	17 Apr 2018
DOIs	https://doi.org/10.1039/c8cc01661e
Publication status	Published - 11 May 2018

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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Cite this

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title = "Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols",

abstract = "Two 'turn on' TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found for TCFCl-GSH and a clear 'turn on' with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.",

author = "Sedgwick, {Adam C.} and Gardiner, {Jordan E.} and Gyoungmi Kim and Maksims Yevglevskis and Lloyd, {Matthew D.} and Jenkins, {A. Toby A.} and Bull, {Steven D.} and Juyoung Yoon and James, {Tony D.}",

year = "2018",

month = may,

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doi = "10.1039/c8cc01661e",

language = "English",

volume = "54",

pages = "4786--4789",

journal = "Chemical Communications",

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publisher = "Royal Society of Chemistry",

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T1 - Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols

AU - Sedgwick, Adam C.

AU - Gardiner, Jordan E.

AU - Kim, Gyoungmi

AU - Yevglevskis, Maksims

AU - Lloyd, Matthew D.

AU - Jenkins, A. Toby A.

AU - Bull, Steven D.

AU - Yoon, Juyoung

AU - James, Tony D.

PY - 2018/5/11

Y1 - 2018/5/11

N2 - Two 'turn on' TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found for TCFCl-GSH and a clear 'turn on' with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.

AB - Two 'turn on' TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found for TCFCl-GSH and a clear 'turn on' with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.

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SN - 1359-7345

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EP - 4789

JO - Chemical Communications

JF - Chemical Communications

IS - 38

ER -

Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols

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Matthew Lloyd

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Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols

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Matthew Lloyd

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