Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity

Reyna Ballim, Yusuke Nagao, Robert N. Kelsh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Neural crest cells are a highly multipotent and migratory cell type that are important for adult pigment pattern formation, cellular homeostasis, and regeneration. The optical transparency and accessibility of fish embryos makes them particularly well-suited to high-resolution analysis of neural crest development. However, the dispersive nature of these cells adds to the challenge of their study. We describe key protocols for the analysis of neural crest development in zebrafish and medaka, including live imaging of neural crest cells and differentiating pigment cells and transient transgenesis assays that can be used to manipulate neural crest development.
Original languageEnglish
Title of host publicationNeural Crest Cells
EditorsQuentin Schwartz, Sophia Wizniak
PublisherSpringer
Chapter15
Pages195-206
Number of pages12
Volume1976
ISBN (Print)9781493994120
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1976

Keywords

  • Iridophore
  • Leucophore
  • Live imaging
  • Medaka
  • Melanocyte
  • Neural crest
  • Pigment cells
  • Tol2
  • Transient transgenesis
  • Xanthophore
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ballim, R., Nagao, Y., & Kelsh, R. N. (2019). Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity. In Q. Schwartz, & S. Wizniak (Eds.), Neural Crest Cells (Vol. 1976, pp. 195-206). (Methods in Molecular Biology; Vol. 1976). Springer. https://doi.org/10.1007/978-1-4939-9412-0_15