Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity

Reyna Ballim; Yusuke Nagao; Robert N. Kelsh

doi:10.1007/978-1-4939-9412-0_15

Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity

Reyna Ballim, Yusuke Nagao, Robert N. Kelsh

University of Bath

Research output: Chapter or section in a book/report/conference proceeding › Chapter or section

1 Citation (SciVal)

Abstract

Neural crest cells are a highly multipotent and migratory cell type that are important for adult pigment pattern formation, cellular homeostasis, and regeneration. The optical transparency and accessibility of fish embryos makes them particularly well-suited to high-resolution analysis of neural crest development. However, the dispersive nature of these cells adds to the challenge of their study. We describe key protocols for the analysis of neural crest development in zebrafish and medaka, including live imaging of neural crest cells and differentiating pigment cells and transient transgenesis assays that can be used to manipulate neural crest development.

Original language	English
Title of host publication	Neural Crest Cells
Editors	Quentin Schwartz, Sophia Wizniak
Publisher	Springer
Chapter	15
Pages	195-206
Number of pages	12
Volume	1976
ISBN (Print)	9781493994120
DOIs	https://doi.org/10.1007/978-1-4939-9412-0_15
Publication status	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	1976

Keywords

Iridophore
Leucophore
Live imaging
Medaka
Melanocyte
Neural crest
Pigment cells
Tol2
Transient transgenesis
Xanthophore
Zebrafish

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-9412-0_15

Cite this

Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity. / Ballim, Reyna; Nagao, Yusuke; Kelsh, Robert N.
Neural Crest Cells. ed. / Quentin Schwartz; Sophia Wizniak. Vol. 1976 Springer, 2019. p. 195-206 (Methods in Molecular Biology; Vol. 1976).

Research output: Chapter or section in a book/report/conference proceeding › Chapter or section

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abstract = "Neural crest cells are a highly multipotent and migratory cell type that are important for adult pigment pattern formation, cellular homeostasis, and regeneration. The optical transparency and accessibility of fish embryos makes them particularly well-suited to high-resolution analysis of neural crest development. However, the dispersive nature of these cells adds to the challenge of their study. We describe key protocols for the analysis of neural crest development in zebrafish and medaka, including live imaging of neural crest cells and differentiating pigment cells and transient transgenesis assays that can be used to manipulate neural crest development.",

keywords = "Iridophore, Leucophore, Live imaging, Medaka, Melanocyte, Neural crest, Pigment cells, Tol2, Transient transgenesis, Xanthophore, Zebrafish",

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AU - Nagao, Yusuke

AU - Kelsh, Robert N.

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AB - Neural crest cells are a highly multipotent and migratory cell type that are important for adult pigment pattern formation, cellular homeostasis, and regeneration. The optical transparency and accessibility of fish embryos makes them particularly well-suited to high-resolution analysis of neural crest development. However, the dispersive nature of these cells adds to the challenge of their study. We describe key protocols for the analysis of neural crest development in zebrafish and medaka, including live imaging of neural crest cells and differentiating pigment cells and transient transgenesis assays that can be used to manipulate neural crest development.

KW - Iridophore

KW - Leucophore

KW - Live imaging

KW - Medaka

KW - Melanocyte

KW - Neural crest

KW - Pigment cells

KW - Tol2

KW - Transient transgenesis

KW - Xanthophore

KW - Zebrafish

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M3 - Chapter or section

SN - 9781493994120

VL - 1976

T3 - Methods in Molecular Biology

SP - 195

EP - 206

BT - Neural Crest Cells

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PB - Springer

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Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

JSPS Fellowship for Y.Nagao

Marie Curie IF Deeya Ballim - SOX10 Mutants

A Systems Biology Approach to Neural Crest Development

MC2-Bioimaging and cell analysis

Cite this

Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

JSPS Fellowship for Y.Nagao

Marie Curie IF Deeya Ballim - SOX10 Mutants

A Systems Biology Approach to Neural Crest Development

Equipment

MC2-Bioimaging and cell analysis

Cite this