Lipoprotein signal peptides are processed by Lsp and Eep of Streptococcus uberis

E L Denham, P N Ward, James A. Leigh

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Lipoprotein signal peptidase (lsp) is responsible for cleaving the signal peptide sequence of lipoproteins in gram-positive bacteria. Investigation of the role of Lsp in Streptococcus uberis, a common cause of bovine mastitis, was undertaken using the lipoprotein MtuA (a protein essential for virulence) as a marker. The S. uberis lsp mutant phenotype displayed novel lipoprotein processing. Not only was full-length (uncleaved) MtuA detected by Western blotting, but during late log phase, a lower-molecular-weight derivative of MtuA was evident. Similar analysis of an S. uberis double mutant containing insertions disrupting both lsp and eep (a homologue of the Enterococcus faecalis "enhanced expression of pheromone" gene) indicated a role for eep in cleavage of lipoproteins in the absence of Lsp. Such a function may indicate a role for eep in maintenance of secretion pathways during disruption of normal lipoprotein processing.

Original languageEnglish
Pages (from-to)4641-4647
Number of pages7
JournalJournal of Bacteriology
Volume190
Issue number13
Early online date20 Jun 2008
DOIs
Publication statusPublished - Jul 2008

Keywords

  • Bacterial Proteins/genetics
  • Blotting, Southern
  • Blotting, Western
  • Computational Biology
  • Genome, Bacterial
  • Lipoproteins/genetics
  • Mutation
  • Protein Sorting Signals/genetics
  • Streptococcus/genetics

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