Lipid nanostructures in butter oil: Structural and physicochemical characterization

Amrutha Arimboor Sunny, Sergey Zlatogorsky, Yogita Patil-Sen, Tamar Garcia-Sorribes, Adam Squires, Chandrashekhar V. Kulkarni

Research output: Chapter or section in a book/report/conference proceedingChapter or section


Butter oil is derived from butter and constitutes of triglycerides along with small amounts of other lipids and fat-soluble components. Food grade lipids can be easily sourced from butter oil which finds a great potential in various ancient, modern as well as emerging applications. Due to almost “all fat” content, butter oil is not soluble in water, but it can be emulsified or combined with other components to enhance its applicability. In order to develop and optimize various applications, it is vital to identify self-assembled nanostructures formed within the butter oil. This report involves nanostructural studies by small (SAXS) and wide (WAXS) angle X-ray scattering and microstructural analysis of butter and butter oil using microscopic techniques. Both butter and butter oil display various polymorphs, detected by WAXS, but in general, the self-assembled nanostructure was identified to be a lamellar phase with the lattice parameter of about 41.8 Å. Physicochemical properties of butter oil, namely solubility, density, thermal behavior, functional groups and molecular structure elucidation also contribute to this report.

Original languageEnglish
Title of host publicationAdvances in Biomembranes and Lipid Self-Assembly
EditorsAleš Iglič, Michael Rappolt, Patricia Losada-Pérez
PublisherElsevier Science Publishers B.V.
Number of pages23
ISBN (Print)9780323985963
Publication statusPublished - 31 Jan 2022

Publication series

NameAdvances in Biomembranes and Lipid Self-Assembly
ISSN (Print)2451-9634


  • Anhydrous milk fat
  • Butter
  • Butter oil
  • Density
  • Ghee
  • Lipid nanostructures
  • NMR spectroscopy
  • Small-angle X-ray scattering

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biochemistry


Dive into the research topics of 'Lipid nanostructures in butter oil: Structural and physicochemical characterization'. Together they form a unique fingerprint.

Cite this