Lineage-specific plasmid acquisition and the evolution of specialized pathogens in Bacillus thuringiensis and the Bacillus cereus group

Guillaume Méric, Leonardos Mageiros, Ben Pascoe, Dan J Woodcock, Evangelos Mourkas, Sarah Lamble, Rory Bowden, Keith A Jolley, Ben Raymond, Samuel K Sheppard

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Bacterial plasmids can vary from small selfish genetic elements to large autonomous replicons that constitute a significant proportion of total cellular DNA. By conferring novel function to the cell, plasmids may facilitate evolution but their mobility may be opposed by co-evolutionary relationships with chromosomes or encouraged via the infectious sharing of genes encoding public goods. Here, we explore these hypotheses through large-scale examination of the association between plasmids and chromosomal DNA in the phenotypically diverse Bacillus cereus group. This complex group is rich in plasmids, many of which encode essential virulence factors (Cry toxins) that are known public goods. We characterized population genomic structure, gene content and plasmid distribution to investigate the role of mobile elements in diversification. We analysed coding sequence within the core and accessory genome of 190 B. cereus group isolates, including 23 novel sequences and genes from 410 reference plasmid genomes. While cry genes were widely distributed, those with invertebrate toxicity were predominantly associated with one sequence cluster (clade 2) and phenotypically defined Bacillus thuringiensis. Cry toxin plasmids in clade 2 showed evidence of recent horizontal transfer and variable gene content, a pattern of plasmid segregation consistent with transfer during infectious cooperation. Nevertheless, comparison between clades suggests that co-evolutionary interactions may drive association between plasmids and chromosomes and limit wider transfer of key virulence traits. Proliferation of successful plasmid and chromosome combinations is a feature of specialized pathogens with characteristic niches (Bacillus anthracis, B. thuringiensis) and has occurred multiple times in the B. cereus group.

Original languageEnglish
Pages (from-to)1524-1540
Number of pages17
JournalMolecular Ecology
Volume27
Issue number7
Early online date6 Mar 2018
DOIs
Publication statusPublished - 1 Apr 2018

Fingerprint

Bacillus thuringiensis
Bacillus cereus
plasmid
plasmids
Plasmids
pathogen
pathogens
gene
chromosome
Chromosomes
virulence
chromosomes
genes
toxin
Genes
toxins
genome
Genome
Bacillus anthracis
DNA

Keywords

  • Bacillus cereus
  • Bacillus thuringiensis
  • insecticidal toxins
  • mobile genetic elements
  • pan-genome

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Lineage-specific plasmid acquisition and the evolution of specialized pathogens in Bacillus thuringiensis and the Bacillus cereus group. / Méric, Guillaume; Mageiros, Leonardos; Pascoe, Ben; Woodcock, Dan J; Mourkas, Evangelos; Lamble, Sarah; Bowden, Rory; Jolley, Keith A; Raymond, Ben; Sheppard, Samuel K.

In: Molecular Ecology, Vol. 27, No. 7, 01.04.2018, p. 1524-1540.

Research output: Contribution to journalArticle

Méric, Guillaume ; Mageiros, Leonardos ; Pascoe, Ben ; Woodcock, Dan J ; Mourkas, Evangelos ; Lamble, Sarah ; Bowden, Rory ; Jolley, Keith A ; Raymond, Ben ; Sheppard, Samuel K. / Lineage-specific plasmid acquisition and the evolution of specialized pathogens in Bacillus thuringiensis and the Bacillus cereus group. In: Molecular Ecology. 2018 ; Vol. 27, No. 7. pp. 1524-1540.
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