Limiting pseudomonas aeruginosa biofilm formation using cold atmospheric pressure plasma

Bethany L. Patenall; Hollie Hathaway; Adam C. Sedgwick; Naing T. Thet; George T. Williams; Amber E. Young; Sarah L. Allinson; Robert D. Short; A. Toby A. Jenkins

doi:10.1615/PLASMAMED.2018028325

Limiting pseudomonas aeruginosa biofilm formation using cold atmospheric pressure plasma

Bethany L. Patenall, Hollie Hathaway, Adam C. Sedgwick, Naing T. Thet, George T. Williams, Amber E. Young, Sarah L. Allinson, Robert D. Short, A. Toby A. Jenkins

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

15 Citations (SciVal)

Abstract

We investigate the ability to disrupt and limit growth biofilms ol Pseudomonas aeruginosa using application of cold atmospheric pressure (CAP) plasma. The effect of the bio-film's exposure to a helium (CAP)jet was assessed at varying time points during biofilm maturation. Results showed that the amount of time during biofilm growth that CAP pressure was applied has a crucial role on the ability of biofilms to mature and recover after CAP exposure. Intervention during the early stages of biofilm formation (0-8 h) results in a 4-5-log reduction in viable bacterial cells (measured at 24 h of incubation) relative to untreated biofilms. However, CAP treatment of biofilm at 12 h and above only results in a 2-log reduction in viable cells. This has potentially important implications for future clinical application of CAP to treat infected wounds.

Original language	English
Pages (from-to)	269-277
Number of pages	9
Journal	Plasma Medicine
Volume	8
Issue number	3
DOIs	https://doi.org/10.1615/PLASMAMED.2018028325
Publication status	Published - 1 Jan 2018

Funding

a Department of Chemistry, University of Bath, UK; bDepartment of Chemistry, Lancaster University, UK; cThe Scar Free Foundation Centre for Children’s Burns Research, The Bristol Royal Hospital for Children, Bristol, UK; dDivision of Biomedical and Life Sciences, Lancaster University, Lancaster, UK The authors thank the Engineering and Physical Sciences Research Council (EPSRC) for Grant No. EP/R003556/1. B.L.P. thanks James Tudor and Alastair and Nathalie Watson for additional funding. G.T.W. is grateful to the EPSRC and Public Health England. A.T.A.J., A.C.S., and N.T.T. thank the EPSRC for funding smart-wound plasma (Grant

Keywords

Biofilm
Plasma
Pseudomonas aeruginosa

ASJC Scopus subject areas

Biomedical Engineering
General Physics and Astronomy

Access to Document

10.1615/PLASMAMED.2018028325

Cite this

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title = "Limiting pseudomonas aeruginosa biofilm formation using cold atmospheric pressure plasma",

abstract = "We investigate the ability to disrupt and limit growth biofilms ol Pseudomonas aeruginosa using application of cold atmospheric pressure (CAP) plasma. The effect of the bio-film's exposure to a helium (CAP)jet was assessed at varying time points during biofilm maturation. Results showed that the amount of time during biofilm growth that CAP pressure was applied has a crucial role on the ability of biofilms to mature and recover after CAP exposure. Intervention during the early stages of biofilm formation (0-8 h) results in a 4-5-log reduction in viable bacterial cells (measured at 24 h of incubation) relative to untreated biofilms. However, CAP treatment of biofilm at 12 h and above only results in a 2-log reduction in viable cells. This has potentially important implications for future clinical application of CAP to treat infected wounds.",

keywords = "Biofilm, Plasma, Pseudomonas aeruginosa",

author = "Patenall, {Bethany L.} and Hollie Hathaway and Sedgwick, {Adam C.} and Thet, {Naing T.} and Williams, {George T.} and Young, {Amber E.} and Allinson, {Sarah L.} and Short, {Robert D.} and Jenkins, {A. Toby A.}",

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AU - Patenall, Bethany L.

AU - Hathaway, Hollie

AU - Sedgwick, Adam C.

AU - Thet, Naing T.

AU - Williams, George T.

AU - Young, Amber E.

AU - Allinson, Sarah L.

AU - Short, Robert D.

AU - Jenkins, A. Toby A.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - We investigate the ability to disrupt and limit growth biofilms ol Pseudomonas aeruginosa using application of cold atmospheric pressure (CAP) plasma. The effect of the bio-film's exposure to a helium (CAP)jet was assessed at varying time points during biofilm maturation. Results showed that the amount of time during biofilm growth that CAP pressure was applied has a crucial role on the ability of biofilms to mature and recover after CAP exposure. Intervention during the early stages of biofilm formation (0-8 h) results in a 4-5-log reduction in viable bacterial cells (measured at 24 h of incubation) relative to untreated biofilms. However, CAP treatment of biofilm at 12 h and above only results in a 2-log reduction in viable cells. This has potentially important implications for future clinical application of CAP to treat infected wounds.

AB - We investigate the ability to disrupt and limit growth biofilms ol Pseudomonas aeruginosa using application of cold atmospheric pressure (CAP) plasma. The effect of the bio-film's exposure to a helium (CAP)jet was assessed at varying time points during biofilm maturation. Results showed that the amount of time during biofilm growth that CAP pressure was applied has a crucial role on the ability of biofilms to mature and recover after CAP exposure. Intervention during the early stages of biofilm formation (0-8 h) results in a 4-5-log reduction in viable bacterial cells (measured at 24 h of incubation) relative to untreated biofilms. However, CAP treatment of biofilm at 12 h and above only results in a 2-log reduction in viable cells. This has potentially important implications for future clinical application of CAP to treat infected wounds.

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KW - Plasma

KW - Pseudomonas aeruginosa

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Limiting pseudomonas aeruginosa biofilm formation using cold atmospheric pressure plasma

Abstract

Funding

Keywords

ASJC Scopus subject areas

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Fingerprint

Smartwound-plasma

Cite this

Limiting pseudomonas aeruginosa biofilm formation using cold atmospheric pressure plasma

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Smartwound-plasma

Cite this