Light induced heme oxygenase 1 is suppressed by Bach1 in human skin keratinocytes

Ruoqi Xu, Wanqian Liu, Jin Zhang, Li Yang, Linhong Deng, Rex M Tyrrell, Julia Li Zhong

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Abstract

Ultraviolet A (UVA) radiation is an oxidizing agent that strongly induces the heme oxygenase 1(HO-1) expression in cultured human skin fibroblasts, but weakly induces it in skin keratinocytes. Here, we report that low basal levels of HO- 1 and much higher basal levels of HO-2 protein were observed in keratinocytes compared with fibroblasts. Silencing of Bach1 strongly increased HO-1 levels in HaCaT transformed keratinocytes and these HO-1 levels were not further increased by either UVA irradiation or silencing of HO-2. This is consistent with the conclusion that high constitutive levels of HO-2 expression in keratinocytes are responsible for the resistance of these cells to HO-1 induction by UVA radiation and that Bach1 plays a predominant role in influencing the lack of HO-1 expression in keratinocytes. Bach1 inhibition reduced the 500 kJ/m2 UVA-induced cell damage by LDH membrane integrity and MTS viability assays. These results suggest that Bach1 inhibition protect against high dose of UVA irradiation induced damage in keratinocytes.
Original languageEnglish
Article number78450Z
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume7845
DOIs
Publication statusPublished - 2010

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