Ligand-independent traffic of notch buffers activated armadillo in Drosophila

Phil G. T. Sanders, Silvia Muñoz-Descalzo, Tina Balayo, Frederik Wirtz-Peitz, Penelope Hayward, Alfonso Martinez Arias

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Abstract

Notch receptors act as ligand-dependent membrane-tethered transcription factors with a prominent role in binary cell fate decisions during development, which is conserved across species. In addition there is increasing evidence for other functions of Notch, particularly in connection with Wnt signalling: Notch is able to modulate the activity of Armadillo/ßcatenin, the effector of Wnt signalling, in a manner that is independent of its transcriptional activity. Here we explore the mechanism of this interaction in the epithelium of the Drosophila imaginal discs and find that it is mediated by the ligand-independent endocytosis and traffic of the Notch receptor. Our results show that Notch associates with Armadillo near the adherens junctions and that it is rapidly endocytosed promoting the traffic of an activated form of Armadillo into endosomal compartments, where it may be degraded. As Notch has the ability to interact with and downregulate activated forms of Armadillo, it is possible that in vivo Notch regulates the transcriptionally competent pool of Armadillo. These interactions reveal a previously unknown activity of Notch, which serves to buffer the function of activated Armadillo and might underlie some of its transcription-independent effects.
Original languageEnglish
Article numbere1000169
Number of pages18
JournalPLoS Biology
Volume7
Issue number8
DOIs
Publication statusPublished - 11 Aug 2009

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Armadillos
Notch Receptors
Dasypodidae
traffic
Drosophila
Buffers
buffers
Ligands
Transcription
Transcription Factors
Endocytosis
Membranes
Imaginal Discs
imaginal discs
Adherens Junctions
receptors
endocytosis
ligands
epithelium
Down-Regulation

Cite this

Sanders, P. G. T., Muñoz-Descalzo, S., Balayo, T., Wirtz-Peitz, F., Hayward, P., & Martinez Arias, A. (2009). Ligand-independent traffic of notch buffers activated armadillo in Drosophila. PLoS Biology, 7(8), [e1000169]. https://doi.org/10.1371/journal.pbio.1000169

Ligand-independent traffic of notch buffers activated armadillo in Drosophila. / Sanders, Phil G. T.; Muñoz-Descalzo, Silvia; Balayo, Tina; Wirtz-Peitz, Frederik; Hayward, Penelope; Martinez Arias, Alfonso.

In: PLoS Biology, Vol. 7, No. 8, e1000169, 11.08.2009.

Research output: Contribution to journalArticle

Sanders, PGT, Muñoz-Descalzo, S, Balayo, T, Wirtz-Peitz, F, Hayward, P & Martinez Arias, A 2009, 'Ligand-independent traffic of notch buffers activated armadillo in Drosophila', PLoS Biology, vol. 7, no. 8, e1000169. https://doi.org/10.1371/journal.pbio.1000169
Sanders PGT, Muñoz-Descalzo S, Balayo T, Wirtz-Peitz F, Hayward P, Martinez Arias A. Ligand-independent traffic of notch buffers activated armadillo in Drosophila. PLoS Biology. 2009 Aug 11;7(8). e1000169. https://doi.org/10.1371/journal.pbio.1000169
Sanders, Phil G. T. ; Muñoz-Descalzo, Silvia ; Balayo, Tina ; Wirtz-Peitz, Frederik ; Hayward, Penelope ; Martinez Arias, Alfonso. / Ligand-independent traffic of notch buffers activated armadillo in Drosophila. In: PLoS Biology. 2009 ; Vol. 7, No. 8.
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