Label-Free Electrochemical Detection of S. Mutans Exploiting Commercially Fabricated Printed Circuit Board Sensing Electrodes

Gorachand Dutta, Abdoulie Jallow, Debjani Paul, Despina Moschou

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This paper reports for the first time printed-circuit-board (PCB)-based label-free electrochemical detection of bacteria. The demonstrated immunosensor was implemented on a PCB sensing platform which was designed and fabricated in a standard PCB manufacturing facility. Bacteria were directly captured on the PCB sensing surface using a specific, pre-immobilized antibody. Electrochemical impedance spectra (EIS) were recorded and used to extract the charge transfer resistance (R ct) value for the different bacteria concentrations under investigation. As a proof-of-concept, Streptococcus mutans (S. mutans) bacteria were quantified in a phosphate buffered saline (PBS) buffer, achieving a limit of detection of 10 3 CFU/mL. Therefore, the proposed biosensor is an attractive candidate for the development of a simple and robust point-of-care diagnostic platform for bacteria identification, exhibiting good sensitivity, high selectivity, and excellent reproducibility.

Original languageEnglish
Article number575
Pages (from-to)1-9
Number of pages9
JournalMicromachines
Volume10
Issue number9
DOIs
Publication statusPublished - 30 Aug 2019

Keywords

  • Direct bacteria detection
  • Electrochemical impedance spectroscopy
  • Immunosensor
  • Lab-on-PCB (printed-circuit-board)
  • Streptococcus mutans (S. mutans)

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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