LAB-ON-PCB DEVICE FOR HANDHELD, RAPID, MOLECULAR SARS-CoV-2 DIAGNOSTIC IN WASTEWATER

Sotirios Papamatthaiou; James Boxall-Clasby; Varun K.S. Kumar; Mirella Di Lorenzo; Julien Reboud; Jonathan M. Cooper; Pedro Estrela; Barbara Kasprzyk-Hordern; Despina Moschou

LAB-ON-PCB DEVICE FOR HANDHELD, RAPID, MOLECULAR SARS-CoV-2 DIAGNOSTIC IN WASTEWATER

Sotirios Papamatthaiou
, James Boxall-Clasby
, Varun K.S. Kumar
, Mirella Di Lorenzo
, Julien Reboud
, Jonathan M. Cooper
, Pedro Estrela
, Barbara Kasprzyk-Hordern
, Despina Moschou

University of Glasgow

Research output: Chapter or section in a book/report/conference proceeding › Chapter in a published conference proceeding

1 Citation (SciVal)

Abstract

The COVID-19 pandemic has caused large suffering worldwide. Individual, point-of-care testing, although effective, is time-consuming, costly and biased towards patients with COVID symptoms. To address this, Wastewater-Based Epidemiology (WBE) was implemented globally as an early-warning system, providing unbiased and comprehensive SARS-CoV-2 testing of whole communities. However, it still relies on expensive and time-consuming lab-based PCR measurements. To fully unravel WBE's potential as a rapid community testing-tool, new low-cost, rapid and reliable sensors are needed. We report the development of an RT-LAMP handheld system, integrating a continuous-flow microfluidic PCB-chip along with low-cost circuitry enabling real-time fluorescence-detection of SARS-CoV-2 within 10 minutes.

Original language	English
Title of host publication	MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	642-643
Number of pages	2
ISBN (Electronic)	9781733419048
Publication status	Published - 27 Oct 2022
Event	26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 - Hybrid, Hangzhou, China Duration: 23 Oct 2022 → 27 Oct 2022

Publication series

Name	MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
Country/Territory	China
City	Hybrid, Hangzhou
Period	23/10/22 → 27/10/22

Bibliographical note

Funding Information:
Financial support was provided by Research England QR GCRF and EPSRC Impact Acceleration

Funding

Financial support was provided by Research England QR GCRF and EPSRC Impact Acceleration

Keywords

COVID-19
Lab-on-Chip
Lab-on-PCB
LAMP
Point-of-Use
rapid test
wastewater-based epidemiology

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering
General Chemistry
Control and Systems Engineering

Cite this

Papamatthaiou, S., Boxall-Clasby, J., Kumar, V. K. S., Di Lorenzo, M., Reboud, J., Cooper, J. M., Estrela, P., Kasprzyk-Hordern, B., & Moschou, D. (2022). LAB-ON-PCB DEVICE FOR HANDHELD, RAPID, MOLECULAR SARS-CoV-2 DIAGNOSTIC IN WASTEWATER. In MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 642-643). (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

LAB-ON-PCB DEVICE FOR HANDHELD, RAPID, MOLECULAR SARS-CoV-2 DIAGNOSTIC IN WASTEWATER. / Papamatthaiou, Sotirios; Boxall-Clasby, James; Kumar, Varun K.S. et al.
MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2022. p. 642-643 (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter or section in a book/report/conference proceeding › Chapter in a published conference proceeding

Papamatthaiou, S, Boxall-Clasby, J, Kumar, VKS, Di Lorenzo, M, Reboud, J, Cooper, JM, Estrela, P , Kasprzyk-Hordern, B & Moschou, D 2022, LAB-ON-PCB DEVICE FOR HANDHELD, RAPID, MOLECULAR SARS-CoV-2 DIAGNOSTIC IN WASTEWATER. in MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 642-643, 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022, Hybrid, Hangzhou, China, 23/10/22.

Papamatthaiou S, Boxall-Clasby J, Kumar VKS, Di Lorenzo M, Reboud J, Cooper JM et al. LAB-ON-PCB DEVICE FOR HANDHELD, RAPID, MOLECULAR SARS-CoV-2 DIAGNOSTIC IN WASTEWATER. In MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2022. p. 642-643. (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Papamatthaiou, Sotirios ; Boxall-Clasby, James ; Kumar, Varun K.S. et al. / LAB-ON-PCB DEVICE FOR HANDHELD, RAPID, MOLECULAR SARS-CoV-2 DIAGNOSTIC IN WASTEWATER. MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2022. pp. 642-643 (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

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abstract = "The COVID-19 pandemic has caused large suffering worldwide. Individual, point-of-care testing, although effective, is time-consuming, costly and biased towards patients with COVID symptoms. To address this, Wastewater-Based Epidemiology (WBE) was implemented globally as an early-warning system, providing unbiased and comprehensive SARS-CoV-2 testing of whole communities. However, it still relies on expensive and time-consuming lab-based PCR measurements. To fully unravel WBE's potential as a rapid community testing-tool, new low-cost, rapid and reliable sensors are needed. We report the development of an RT-LAMP handheld system, integrating a continuous-flow microfluidic PCB-chip along with low-cost circuitry enabling real-time fluorescence-detection of SARS-CoV-2 within 10 minutes.",

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note = "Funding Information: Financial support was provided by Research England QR GCRF and EPSRC Impact Acceleration ; 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 ; Conference date: 23-10-2022 Through 27-10-2022",

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AU - Kumar, Varun K.S.

AU - Di Lorenzo, Mirella

AU - Reboud, Julien

AU - Cooper, Jonathan M.

AU - Estrela, Pedro

AU - Kasprzyk-Hordern, Barbara

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AB - The COVID-19 pandemic has caused large suffering worldwide. Individual, point-of-care testing, although effective, is time-consuming, costly and biased towards patients with COVID symptoms. To address this, Wastewater-Based Epidemiology (WBE) was implemented globally as an early-warning system, providing unbiased and comprehensive SARS-CoV-2 testing of whole communities. However, it still relies on expensive and time-consuming lab-based PCR measurements. To fully unravel WBE's potential as a rapid community testing-tool, new low-cost, rapid and reliable sensors are needed. We report the development of an RT-LAMP handheld system, integrating a continuous-flow microfluidic PCB-chip along with low-cost circuitry enabling real-time fluorescence-detection of SARS-CoV-2 within 10 minutes.

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KW - Lab-on-Chip

KW - Lab-on-PCB

KW - LAMP

KW - Point-of-Use

KW - rapid test

KW - wastewater-based epidemiology

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AN - SCOPUS:85175736579

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BT - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences

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T2 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022

Y2 - 23 October 2022 through 27 October 2022

ER -

LAB-ON-PCB DEVICE FOR HANDHELD, RAPID, MOLECULAR SARS-CoV-2 DIAGNOSTIC IN WASTEWATER

Abstract

Publication series

Conference

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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