Knockdown of nuclear-located enhancer RNAs and long ncRNAs using locked nucleic acid gapmers

Benoit T. Roux, Mark A. Lindsay, James A. Heward

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The human genome is widely transcribed outside of protein-coding genes, producing thousands of noncoding RNAs from different subfamilies including enhancer RNAs. Functional studies to determine the role of individual genes are challenging with noncoding RNAs appearing to be more difficult to knockdown than mRNAs. One factor that may have hindered progress is that the majority of noncoding RNAs are thought to be located within the nucleus, where the efficiency of traditional RNA interference techniques is debatable. Here we present an alternative RNA interference technique utilizing Locked Nucleic Acids, which is able to efficiently knockdown noncoding RNAs irrespective of intracellular location.

Original languageEnglish
Pages (from-to)11-18
Number of pages8
JournalMethods in Molecular Biology
Volume1468
Early online date24 Sep 2016
DOIs
Publication statusPublished - 31 Dec 2017

Keywords

  • Enhancer
  • ERNA
  • GapmeR
  • LNA
  • Long ncRNA
  • Nuclear
  • RNAi

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this